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Literature summary for 1.14.13.148 extracted from

  • Chen, Y.; Patel, N.A.; Crombie, A.; Scrivens, J.H.; Murrell, J.C.
    Bacterial flavin-containing monooxygenase is trimethylamine monooxygenase (2011), Proc. Natl. Acad. Sci. USA, 108, 17791-17796.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.148

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BLR(DE3) pLysS cells Ruegeria pomeroyi
expressed in Escherichia coli BLR(DE3) pLysS cells Roseovarius sp.
expressed in Escherichia coli BLR(DE3) pLysS cells Candidatus Pelagibacter ubique
expressed in Escherichia coli BLR(DE3) pLysS cells Methylocella silvestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
0.0103
-
 Dimethylsulfide recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
0.0208
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Ruegeria pomeroyi
0.0216
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Roseovarius sp.
0.0275
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Candidatus Pelagibacter ubique
0.0282
-
 Methimazole recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
0.0285
-
 N,N,N-trimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Candidatus Pelagibacter ubique
0.0357
-
 dimethylaniline recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
0.0897
-
 N,N-dimethylamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
3.139
-
 cysteamine recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris
3.575
-
 DMSO recombinant enzyme, in 80 mM PIPES buffer (pH 7.6), at 22°C Methylocella silvestris

Organism

Organism UniProt Comment Textmining
Candidatus Pelagibacter ubique
-
-
-
Candidatus Pelagibacter ubique HTCC1002
-
-
-
Candidatus Pelagibacter ubique HTCC7211
-
-
-
Methylocella silvestris
-
-
-
no activity in Dinoroseobacter shibae
-
-
-
no activity in Oceanicola batsensis
-
-
-
no activity in Roseobacter sp.
-
-
-
no activity in Roseobacter sp. SK209-2-6
-
-
-
no activity in Sagittula stellata
-
-
-
Roseovarius sp.
-
-
-
Roseovarius sp. 217
-
-
-
Ruegeria pomeroyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cysteamine + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
dimethylaniline + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
dimethylsulfide + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
DMSO + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
methimazole + NADPH + H+ + O2
-
 Methylocella silvestris ?
-
 ?
additional information no activity with N-monomethylamine, glutathione, and cysteine Methylocella silvestris ?
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Ruegeria pomeroyi N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Roseovarius sp. N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter ubique N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Methylocella silvestris N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter ubique HTCC7211 N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter ubique HTCC1002 N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N,N-trimethylamine + NADPH + H+ + O2
-
 Roseovarius sp. 217 N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N-dimethylamine + NADPH + H+ + O2
-
 Methylocella silvestris N,N-dimethylamine N-oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
flavin-containing monooxygenase
-
 Ruegeria pomeroyi
flavin-containing monooxygenase
-
 Roseovarius sp.
flavin-containing monooxygenase
-
 Candidatus Pelagibacter ubique
flavin-containing monooxygenase
-
 Methylocella silvestris
FMO
-
 Ruegeria pomeroyi
FMO
-
 Roseovarius sp.
FMO
-
 Candidatus Pelagibacter ubique
FMO
-
 Methylocella silvestris
TMA monooxygenase
-
 Ruegeria pomeroyi
TMA monooxygenase
-
 Roseovarius sp.
TMA monooxygenase
-
 Candidatus Pelagibacter ubique
TMA monooxygenase
-
 Methylocella silvestris
TMM
-
 Ruegeria pomeroyi
TMM
-
 Roseovarius sp.
TMM
-
 Candidatus Pelagibacter ubique
TMM
-
 Methylocella silvestris

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Ruegeria pomeroyi
FAD
-
 Roseovarius sp.
FAD
-
 Candidatus Pelagibacter ubique
FAD
-
 Methylocella silvestris
NADPH required for activity Ruegeria pomeroyi
NADPH required for activity Roseovarius sp.
NADPH required for activity Candidatus Pelagibacter ubique
NADPH required for activity Methylocella silvestris

Expression

Organism Comment Expression
Methylocella silvestris the enzyme is absent during growth on methanol additional information
Methylocella silvestris the enzyme is highly expressed in N,N,N-trimethylamine-grown Methylocella silvestris up