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Literature summary for 1.14.13.148 extracted from
- Cloning and sequencing of flavin-containing monooxygenases FMO3 and FMO4 from rabbit and characterization of FMO3 (1994), J. Biol. Chem., 269, 14314-14322.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | n-octylamine has no effect on isoform FMO3 | Oryctolagus cuniculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae strain 334 and in Escherichia coli JM109 cells | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chlorpromazine | - |
Oryctolagus cuniculus |  |
| prochlorperazine | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
Methimazole | isoform FMO3, at pH 8.4, temperature not specified in the publication | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59815 | - |
59815, isofom FMO3, calculated from amino acid sequence | Oryctolagus cuniculus |
| 62992 | - |
x * 62992, isoform FMO4, calculated from amino acid sequence | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | P32417 | isoform FMO3 | - |
| Oryctolagus cuniculus | P36367 | isoform FMO4 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | isoform FMO4 is most prominent in the kidney | Oryctolagus cuniculus | - |
| liver | isoform FMO3 is most prominent in the liver | Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.025 | - |
isoform FMO3, using 1 mM methimazole as substrate, at pH 8.4, temperature not specified in the publication | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methimazole + NADPH + H+ + O2 | - |
Oryctolagus cuniculus | methimazole N-oxide + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 59815, isofom FMO3, calculated from amino acid sequence | Oryctolagus cuniculus |
| ? | x * 62992, isoform FMO4, calculated from amino acid sequence | Oryctolagus cuniculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EC 1.14.13.8 | assigned to | Oryctolagus cuniculus |
| flavin-containing monooxygenase | - |
Oryctolagus cuniculus |
| FMO3 | isoform | Oryctolagus cuniculus |
| FMO4 | isoform | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Oryctolagus cuniculus | |
| NADPH | required for activity | Oryctolagus cuniculus | |
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