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Literature summary for 1.14.13.148 extracted from

  • Strøm, A.
    Biosynthesis of trimethylamine oxide in Calanus finmarchicus. Properties of a soluble trimethylamine monooxygenase (1980), Comp. Biochem. Physiol. B, 65, 243-249.
No PubMed abstract available
Search for more literature for 1.14.13.148

Activating Compound

Activating Compound Comment Organism Structure
O2 when the oxygen in the enzyme assay mixture is replaced with 100% nitrogen, there is no formation of N,N,N-trimethylamine N-oxide, showing that the enzyme is oxygen-dependent Calanus finmarchicus

General Stability

General Stability Organism
addition of FAD and dithiothreitol together with NADPH improves the stability of the enzyme Calanus finmarchicus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.2 mM Cu2+ inhibits the enzyme strongly without any preincubation Calanus finmarchicus
additional information 10 mM EDTA, 1 mM potassium cyanide and 1 mM sodium azide do not inhibit the enzyme. Bubbling the enzyme solution with CO for 2 min at 0°C or including CO (CO:O2 ratio 1:1) during the tests has no effect on enzyme activity Calanus finmarchicus
N-ethylmaleimide preincubation of the enzyme for 15 min at 14°C, pH 7.7, in the presence of 1.0 mM N-ethylmaleimide inhibits the enzymatic activity approximately by 25% Calanus finmarchicus
p-chloromercuribenzoate preincubation of the enzyme for 15 min at 14°C, pH 7.7, in the presence of 0.2 mM p-chloromercuribenzoate inhibits the enzymic activity approximately by 90% Calanus finmarchicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 N,N,N-trimethylamine in potassium diphosphate buffer (pH 7.7), at 14°C Calanus finmarchicus
0.0008
-
 NADPH in potassium diphosphate buffer (pH 7.7), at 14°C Calanus finmarchicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Calanus finmarchicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion activators are found Calanus finmarchicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 gel filtration Calanus finmarchicus

Organism

Organism UniProt Comment Textmining
Calanus finmarchicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-200 gel filtration, DEAE-cellulose column chromatography, and calcium phosphate chromatography Calanus finmarchicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00047
-
 supernatant of homogenate, in potassium diphosphate buffer (pH 7.7), at 14°C Calanus finmarchicus
0.085
-
 after 180fold purification, in potassium diphosphate buffer (pH 7.7), at 14°C Calanus finmarchicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N,N-trimethylamine + NADPH + H+ + O2 highest affinity substrate Calanus finmarchicus N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N-dimethylamine + NADPH + H+ + O2
-
 Calanus finmarchicus N,N-dimethylamine N-oxide + NADP+ + H2O
-
 ?
N,N-dimethylaniline + NADPH + H+ + O2
-
 Calanus finmarchicus N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?
N,N-dimethylbutylamine + NADPH + H+ + O2
-
 Calanus finmarchicus N,N-dimethylbutylamine N-oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
TMA monooxygenase
-
 Calanus finmarchicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
14
-
-
 Calanus finmarchicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 20
-
 Calanus finmarchicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 20 the enzyme is stable for 30 min at temperatures between 0 and 20°C, when the enzyme is preincubated for 30 min at temperatures above 20°C, a decrease in enzymic activity ensues Calanus finmarchicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 7.8
-
 Calanus finmarchicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Calanus finmarchicus
NADPH dependent on, in the absence of NADPH the enzyme is rapidly and irreversibly inactivated at 0-5°C. NADP+ also stabilizes the enzyme, but to a lesser extent than NADPH. No oxidation of N,N,N-trimethylamine is observed when NADH is used in the enzyme assay instead of NADPH Calanus finmarchicus