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Literature summary for 1.14.13.128 extracted from

  • GlŘck, M.; Lingens, F.
    Heteroxanthinedemethylase, a new enzyme in the degradation of caffeine by Pseudomonas putida (1988), Appl. Microbiol. Biotechnol., 28, 59-62.
No PubMed abstract available

General Stability

General Stability Organism
stabilization can be achieved by addition of either glycerol (20%), bovine serum albumin (10 mg/ml), dithiothreitol (1 mM) or the substrate 7-methylxanthine (1 mM). Storage under nitrogen atmosphere has a stabilizing influence. The addition of caffeine (1 mM), dimethylxanthines (1 mM) and protease inhibitors (pepstatin A, PMSF, 0.1 mM) is without influence on stability Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.2 mM, complete inhibition Pseudomonas putida
Co2+ 0.2 mM, 40% inhibition Pseudomonas putida
Cu2+ 0.2 mM, complete inhibition Pseudomonas putida
Fe2+ 1 mM, 37% inhibition Pseudomonas putida
Hg2+ 1 mM, 98% inhibition Pseudomonas putida
Sn2+ 0.2 mM, 17% inhibition Pseudomonas putida
Zn2+ 0.2 mM, complete inhibition Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.113
-
O2 pH and temperature not specified in the publication Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, activation to 123% of control Pseudomonas putida
Mg2+ 1 mM, activation to 132% of control Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas putida

Storage Stability

Storage Stability Organism
4┬░C, pH 6.0, 94% loss of activity after 24 h Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methylxanthine + O2 + NADH + H+
-
Pseudomonas putida xanthine + NAD+ + H2O + formaldehyde
-
?
7-methylxanthine + O2 + NADPH + H+
-
Pseudomonas putida xanthine + NADP+ + H2O + formaldehyde
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30 35
-
Pseudomonas putida

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
45
-
1 min, complete inactivation Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Pseudomonas putida