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Literature summary for 1.14.13.122 extracted from

  • Nagata, N.; Satoh, S.; Tanaka, R.; Tanaka, A.
    Domain structures of chlorophyllide a oxygenase of green plants and Prochlorothrix hollandica in relation to catalytic functions (2004), Planta, 218, 1019-1025.
    View publication on PubMed
Search for more literature for 1.14.13.122

Cloned(Commentary)

Cloned (Comment) Organism
single-copy CAO gene, amino aid sequence and N-terminal extensions comparison with other higher plant sequences and the sequence of the algae Prochlorothrix hollandica, functional expression of ABC, BC, and C domain constructs in Synechocystis sp. PCC6803 reveals that the C domain is sufficient for catalytic activity Arabidopsis thaliana
single-copy CAO gene, DNA and amino acid sequence determination and analysis, amino aid sequence and N-terminal extensions comparison with higher plant sequences, e.g. of Arabidopsis thaliana, functional expression in Synechocystis sp. PCC6803 Prochlorothrix hollandica

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear iron-binding site at the C-terminal C domain, required for activity Arabidopsis thaliana
Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear iron-binding site, required for activity Prochlorothrix hollandica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40830
-
 x * 40830, amino acid sequence calculation Prochlorothrix hollandica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-hydroxychlorophyllide a + O2 + NADPH Prochlorothrix hollandica
-
 chlorophyllide b + H2O + NADP+
-
 ?
7-hydroxychlorophyllide a + O2 + NADPH Arabidopsis thaliana
-
 chlorophyllide b + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+ Prochlorothrix hollandica
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+ Arabidopsis thaliana
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
additional information Arabidopsis thaliana the enzyme is responsible for synthesis of chlorophyllide b which is a photosynthetic antenna pigment required for regulation of antenna size, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9MBA1 single-copy CAO gene
-
Prochlorothrix hollandica
-
 single-copy CAO gene
-

Reaction

Reaction Comment Organism Reaction ID
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+ reaction mechanism, the enzyme C-terminal C domain is sufficient for catalytic activity Arabidopsis thaliana
a
chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+ reaction mechanism, the enzyme C-terminal C domain is sufficient for catalytic activity Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-hydroxychlorophyllide a + O2 + NADPH
-
 Prochlorothrix hollandica chlorophyllide b + H2O + NADP+
-
 ?
7-hydroxychlorophyllide a + O2 + NADPH
-
 Arabidopsis thaliana chlorophyllide b + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+
-
 Prochlorothrix hollandica 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
chlorophyllide a + O2 + NADPH + H+
-
 Arabidopsis thaliana 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
additional information the enzyme is responsible for synthesis of chlorophyllide b which is a photosynthetic antenna pigment required for regulation of antenna size, overview Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40830, amino acid sequence calculation Prochlorothrix hollandica
More the enzyme consists of 3 domains, the N-terminal A domain, the B domain, and the C-terminal C domain Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
CAO
-
 Prochlorothrix hollandica
CAO
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Prochlorothrix hollandica
NADPH
-
 Arabidopsis thaliana