Cloned (Comment) | Organism |
---|---|
single-copy CAO gene, amino aid sequence and N-terminal extensions comparison with other higher plant sequences and the sequence of the algae Prochlorothrix hollandica, functional expression of ABC, BC, and C domain constructs in Synechocystis sp. PCC6803 reveals that the C domain is sufficient for catalytic activity | Arabidopsis thaliana |
single-copy CAO gene, DNA and amino acid sequence determination and analysis, amino aid sequence and N-terminal extensions comparison with higher plant sequences, e.g. of Arabidopsis thaliana, functional expression in Synechocystis sp. PCC6803 | Prochlorothrix hollandica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear iron-binding site at the C-terminal C domain, required for activity | Arabidopsis thaliana | |
Fe2+ | the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear iron-binding site, required for activity | Prochlorothrix hollandica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40830 | - |
x * 40830, amino acid sequence calculation | Prochlorothrix hollandica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-hydroxychlorophyllide a + O2 + NADPH | Prochlorothrix hollandica | - |
chlorophyllide b + H2O + NADP+ | - |
? | |
7-hydroxychlorophyllide a + O2 + NADPH | Arabidopsis thaliana | - |
chlorophyllide b + H2O + NADP+ | - |
? | |
chlorophyllide a + O2 + NADPH + H+ | Prochlorothrix hollandica | - |
7-hydroxychlorophyllide a + H2O + NADP+ | - |
? | |
chlorophyllide a + O2 + NADPH + H+ | Arabidopsis thaliana | - |
7-hydroxychlorophyllide a + H2O + NADP+ | - |
? | |
additional information | Arabidopsis thaliana | the enzyme is responsible for synthesis of chlorophyllide b which is a photosynthetic antenna pigment required for regulation of antenna size, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9MBA1 | single-copy CAO gene | - |
Prochlorothrix hollandica | - |
single-copy CAO gene | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+ | reaction mechanism, the enzyme C-terminal C domain is sufficient for catalytic activity | Arabidopsis thaliana | |
chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+ | reaction mechanism, the enzyme C-terminal C domain is sufficient for catalytic activity | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-hydroxychlorophyllide a + O2 + NADPH | - |
Prochlorothrix hollandica | chlorophyllide b + H2O + NADP+ | - |
? | |
7-hydroxychlorophyllide a + O2 + NADPH | - |
Arabidopsis thaliana | chlorophyllide b + H2O + NADP+ | - |
? | |
chlorophyllide a + O2 + NADPH + H+ | - |
Prochlorothrix hollandica | 7-hydroxychlorophyllide a + H2O + NADP+ | - |
? | |
chlorophyllide a + O2 + NADPH + H+ | - |
Arabidopsis thaliana | 7-hydroxychlorophyllide a + H2O + NADP+ | - |
? | |
additional information | the enzyme is responsible for synthesis of chlorophyllide b which is a photosynthetic antenna pigment required for regulation of antenna size, overview | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40830, amino acid sequence calculation | Prochlorothrix hollandica |
More | the enzyme consists of 3 domains, the N-terminal A domain, the B domain, and the C-terminal C domain | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
CAO | - |
Prochlorothrix hollandica |
CAO | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Prochlorothrix hollandica | |
NADPH | - |
Arabidopsis thaliana |