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Literature summary for 1.14.13.101 extracted from

  • Wang, L.; Beuerle, T.; Timbilla, J.; Ober, D.
    Independent recruitment of a flavin-dependent monooxygenase for safe accumulation of sequestered pyrrolizidine alkaloids in grasshoppers and moths (2012), PLoS ONE, 7, e31796.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Zonocerus variegatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00108
-
senecionine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.00983
-
atropine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.01193
-
monocrotaline in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.2639
-
heliotrine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.8376
-
phalaenopsine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47793
-
2 * 47793, calculated from amino acid sequence Zonocerus variegatus
91200
-
gel filtration Zonocerus variegatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
senecionine + NADPH + H+ + O2 Zonocerus variegatus most efficient substrate senecionine N-oxide + NADP+ + H2O
-
?
seneciphylline + NADPH + H+ + O2 Zonocerus variegatus second best substrate seneciphylline N-oxide + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Zonocerus variegatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography Zonocerus variegatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
atropine + NADPH + H+ + O2
-
Zonocerus variegatus atropine N-oxide + NADP+ + H2O
-
?
axillaridine + NADPH + H+ + O2
-
Zonocerus variegatus axillaridine N-oxide + NADP+ + H2O
-
?
axillarine + NADPH + H+ + O2
-
Zonocerus variegatus axillarine N-oxide + NADP+ + H2O
-
?
cysteamine + NADPH + H+ + O2
-
Zonocerus variegatus cysteamine N-oxide + NADP+ + H2O
-
?
heliotrine + NADPH + H+ + O2
-
Zonocerus variegatus heliotrine N-oxide + NADP+ + H2O
-
?
indicine + NADPH + H+ + O2
-
Zonocerus variegatus indicine N-oxide + NADP+ + H2O
-
?
monocrotaline + NADPH + H+ + O2
-
Zonocerus variegatus monocrotaline N-oxide + NADP+ + H2O
-
?
additional information no activity with senkirkine, heliotridine, supinidine, ephedrine, dimethylaniline, L-cysteine, hydroxylamine, and glutathione Zonocerus variegatus ?
-
?
nicotine + NADPH + H+ + O2
-
Zonocerus variegatus nicotine N-oxide + NADP+ + H2O
-
?
phalaenopsine + NADPH + H+ + O2
-
Zonocerus variegatus phalaenopsine N-oxide + NADP+ + H2O
-
?
retronecine + NADPH + H+ + O2
-
Zonocerus variegatus retronecine N-oxide + NADP+ + H2O
-
?
retrorsine + NADPH + H+ + O2
-
Zonocerus variegatus retrorsine N-oxide + NADP+ + H2O
-
?
rinderine + NADPH + H+ + O2
-
Zonocerus variegatus rinderine N-oxide + NADP+ + H2O
-
?
sarracine + NADPH + H+ + O2
-
Zonocerus variegatus sarracine N-oxide + NADP+ + H2O
-
?
senecionine + NADPH + H+ + O2 most efficient substrate Zonocerus variegatus senecionine N-oxide + NADP+ + H2O
-
?
seneciphylline + NADPH + H+ + O2 second best substrate Zonocerus variegatus seneciphylline N-oxide + NADP+ + H2O
-
?
senecivernine + NADPH + H+ + O2
-
Zonocerus variegatus senecivernine N-oxide + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 47793, calculated from amino acid sequence Zonocerus variegatus

Synonyms

Synonyms Comment Organism
PA N-oxygenase
-
Zonocerus variegatus
PNO
-
Zonocerus variegatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Zonocerus variegatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
heliotrine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.13
-
phalaenopsine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.37
-
atropine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.96
-
monocrotaline in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.99
-
senecionine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
in potassium phosphate buffer (10 mM), Tris/HCl buffer (10 mM), and in glycine/NaOH buffer (100 mM) Zonocerus variegatus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Zonocerus variegatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.155
-
phalaenopsine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
0.454
-
heliotrine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
37.64
-
atropine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
80.5
-
monocrotaline in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus
916.7
-
senecionine in 100 mM glycine/NaOH buffer pH 9.0, at 37°C Zonocerus variegatus