Cloned (Comment) | Organism |
---|---|
expression in active form in Escherichia coli BL21 | Tyria jacobaeae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Tyria jacobaeae | the enzyme allows the larvae to feed on pyrrolizidine alkaloid-containing plants and to accumulate predation-deterrent pyrrolizidine alkaloids in the hemolymph | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tyria jacobaeae | Q8MP06 | sinnabar moth | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemolymph | - |
Tyria jacobaeae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
axillarine + NADPH + O2 | 74% of the activity with senecionine, native enzyme. 83% of the activity with senecionine, recombinant enzyme | Tyria jacobaeae | axillarine N-oxide + NADP+ + H2O | - |
? | |
heliotrine + NADPH + O2 | 25% of the activity with senecionine, native enzyme. 49% of the activity with senecionine, recombinant enzyme | Tyria jacobaeae | heliotrine N-oxide + NADP+ + H2O | - |
? | |
monocrotaline + NADPH + O2 | 92% of the activity with senecionine, native enzyme. 94% of the activity with senecionine, recombinant enzyme | Tyria jacobaeae | monocrotaline N-oxide + NADP+ + H2O | - |
? | |
additional information | the enzyme is highly specific for toxic pyrrolizidine alkaloids. No activity with: senkirkine, dimethylaniline, L-Pro, caffeine, atropine, supinidine, retronecine, phalaenopsine | Tyria jacobaeae | ? | - |
? | |
additional information | the enzyme allows the larvae to feed on pyrrolizidine alkaloid-containing plants and to accumulate predation-deterrent pyrrolizidine alkaloids in the hemolymph | Tyria jacobaeae | ? | - |
? | |
senecionine + NADPH + O2 | - |
Tyria jacobaeae | senecionine N-oxide + NADP+ + H2O | - |
? | |
seneciphylline + NADPH + O2 | 95% of the activity with senecionine, native enzyme. 93% of the activity with senecionine, recombinant enzyme | Tyria jacobaeae | seneciphylline N-oxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
oxygenase, senecionine N- | - |
Tyria jacobaeae |
senecionine N-oxygenase | - |
Tyria jacobaeae |
SNO | - |
Tyria jacobaeae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Tyria jacobaeae |