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Literature summary for 1.14.13.1 extracted from

  • Ambrose, K.V.; Tian, Z.; Wang, Y.; Smith, J.; Zylstra, G.; Huang, B.; Belanger, F.C.
    Functional characterization of salicylate hydroxylase from the fungal endophyte Epichloe festucae (2015), Sci. Rep., 5, 10939 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells Epichloe festucae

Organism

Organism UniProt Comment Textmining
Epichloe festucae A0A0A1E7Z2 isolate Rose City
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Epichloe festucae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoic acid + NADH + H+ + O2 146% activity compared to salicylate Epichloe festucae 2,5-dihydroxyphenol + NAD+ + H2O + CO2
-
?
salicylate + NADH + H+ + O2 100% activity Epichloe festucae catechol + NAD+ + H2O + CO2
-
?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Epichloe festucae

Synonyms

Synonyms Comment Organism
Efe-shyA
-
Epichloe festucae
salicylate hydroxylase
-
Epichloe festucae

Cofactor

Cofactor Comment Organism Structure
NADH
-
Epichloe festucae