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Literature summary for 1.14.13.1 extracted from

  • Uemura, T.; Kita, A.; Watanabe, Y.; Adachi, M.; Kuroki, R.; Morimoto, Y.
    The catalytic mechanism of decarboxylative hydroxylation of salicylate hydroxylase revealed by crystal structure analysis at 2.5 A resolution (2016), Biochem. Biophys. Res. Commun., 469, 158-163 .
    View publication on PubMed
Search for more literature for 1.14.13.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with FAD, sitting drop vapor diffusion method, using 1.26 M ammonium sulfate, 200 mM lithium sulfate and 100 mM Tris-HCl buffer at pH 8.5 Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
salicylate + NADH + H+ + O2 Pseudomonas putida
-
 catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 Pseudomonas putida S-1
-
 catechol + NAD+ + H2O + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida S-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
salicylate + NADH + H+ + O2
-
 Pseudomonas putida catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas putida S-1 catechol + NAD+ + H2O + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
SALH
-
 Pseudomonas putida
salicylate hydroxylase
-
 Pseudomonas putida
salicylate, NADH: oxygen oxidoreductase (1-hydroxylating, decarboxylating)
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas putida
NADH
-
 Pseudomonas putida