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Literature summary for 1.14.13.1 extracted from

  • Huang, S.T.; Teng, C.J.; Lee, Y.H.; Wu, J.Y.; Wang, K.L.; Lin, C.M.
    Design and synthesis of a long-wavelength latent fluorogenic substrate for salicylate hydroxylase: a useful fluorimetric indicator for analyte determination by dehydrogenase-coupled biosensors (2010), Anal. Chem., 82, 7329-7334.
    View publication on PubMed

Application

Application Comment Organism
analysis usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses Pseudomonas sp.
diagnostics usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
salicylate pH 8.0, 37°C Pseudomonas sp.
0.0042
-
4-(3-benzothiazol-2-yl-4-cyano-2-oxo-2H-chromen-7-yloxymethyl)-2-hydroxy-benzoic acid pH 8.0, 37°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
salicylate + O2 + NADH + 2 H+ Pseudomonas sp.
-
catechol + CO2 + H2O + NAD+
-
?
salicylate + O2 + NADPH + 2 H+ Pseudomonas sp.
-
catechol + CO2 + H2O + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(3-benzothiazol-2-yl-4-cyano-2-oxo-2H-chromen-7-yloxymethyl)-2-hydroxy-benzoic acid + O2 + NADH + 2 H+ i.e. SHLF, two-step synthesis of a long-wavelength latent fluorogenic substrate SHLF. In the presence of NADH and under aerobic conditions, SHL catalyzes the decarboxylative hydroxylation of SHLF followed by a quinonemethide-type rearrangement reaction concomitant with the ejection of a fluorescence coumarin 2, which is spontaneous and irreversible at physiological temperatures in aqueous media. The fluorescence signal generated by this process is specific and, in the near red spectral region with an emission maximum at 595 nm, is suppressed by salicylic acid Pseudomonas sp. ?
-
?
salicylate + O2 + NADH + 2 H+
-
Pseudomonas sp. catechol + CO2 + H2O + NAD+
-
?
salicylate + O2 + NADPH + 2 H+
-
Pseudomonas sp. catechol + CO2 + H2O + NADP+
-
?

Synonyms

Synonyms Comment Organism
salicylate hydroxylase
-
Pseudomonas sp.
SHL
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00075
-
4-(3-benzothiazol-2-yl-4-cyano-2-oxo-2H-chromen-7-yloxymethyl)-2-hydroxy-benzoic acid pH 8.0, 37°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas sp.
NADPH
-
Pseudomonas sp.