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Literature summary for 1.14.12.7 extracted from
- The bridging aspartate 178 in phthalate dioxygenase facilitates interactions between the Rieske center and the iron(II)--mononuclear center (2006), Biochemistry, 45, 10208-10216.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D178A | mutation affects protonation of the Rieske center histidine and conformation of subunits within the PDO multimer to create a more open structure with more solvent-accessible Rieske centers | Burkholderia cepacia |
| D178N | mutation affects protonation of the Rieske center histidine and conformation of subunits within the PDO multimer to create a more open structure with more solvent-accessible Rieske centers | Burkholderia cepacia |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | contains 2.85 Fe per monomer | Burkholderia cepacia |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
6 * 48000, gas phase electrophoretic mobility molecular analysis and electrospray ionization mass spectrometry | Burkholderia cepacia |
| 290000 | - |
SDS-PAGE | Burkholderia cepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
- |
- |
| Burkholderia cepacia DB01 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phthalate + NADH + O2 | - |
Burkholderia cepacia | cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O | - |
? | |
| phthalate + NADH + O2 | - |
Burkholderia cepacia DB01 | cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 48000, gas phase electrophoretic mobility molecular analysis and electrospray ionization mass spectrometry | Burkholderia cepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDO | - |
Burkholderia cepacia |
| phthalate dioxygenase | - |
Burkholderia cepacia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
thermally stable at 60°C | Burkholderia cepacia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Burkholderia cepacia | |
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Release 2023.1 (January 2023)
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