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Literature summary for 1.14.12.23 extracted from

  • Ju, K.S.; Parales, R.E.
    Control of substrate specificity by active-site residues in nitrobenzene dioxygenase (2006), Appl. Environ. Microbiol., 72, 1817-1824.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.23

Cloned(Commentary)

Cloned (Comment) Organism
-
 Comamonas sp.

Protein Variants

Protein Variants Comment Organism
F293H mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene Comamonas sp.
F293I mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene Comamonas sp.
F293Q mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene. Mutant is 2.5 times faster than wild-type at oxidizing 2,6-dinitrotoluene while retaining a similar Km for the substrate Comamonas sp.
I350F mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene. Most mononitrotoluene oxidation products formed by the N258V mutant are nitrobenzyl alcohols rather than catechols Comamonas sp.
I350T mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene Comamonas sp.
N258V mutation in alpha-subunit of oxygenase, residue is important for determining regiospecificity with nitroarene substrates and enantiospecificity with naphthalene. Up to 99% of the mononitrotoluene oxidation products formed by the N258V mutant are nitrobenzyl alcohols rather than catechols Comamonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 nitrobenzene wild-type, pH 7.3, 30°C Comamonas sp.
0.012
-
 2,6-dinitrotoluene mutant F293H, pH 7.3, 30°C Comamonas sp.
0.0239
-
 nitrobenzene mutant F293H, pH 7.3, 30°C Comamonas sp.
0.0607
-
 nitrobenzene mutant F293Q, pH 7.3, 30°C Comamonas sp.
0.159
-
 nitrobenzene mutant I350T, pH 7.3, 30°C Comamonas sp.
0.288
-
 2,6-dinitrotoluene mutant F293I, pH 7.3, 30°C Comamonas sp.
0.717
-
 2,6-dinitrotoluene mutant F293Q, pH 7.3, 30°C Comamonas sp.
0.808
-
 2,6-dinitrotoluene mutant N258V, pH 7.3, 30°C Comamonas sp.
0.851
-
 2,6-dinitrotoluene wild-type, pH 7.3, 30°C Comamonas sp.
1.78
-
 nitrobenzene mutant N258V, pH 7.3, 30°C Comamonas sp.

Organism

Organism UniProt Comment Textmining
Comamonas sp. Q8RTL4 oxygenase alpha-subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrotoluene + NADH + O2
-
 Comamonas sp. 4-methyl-5-nitrocatechol + nitrite + NAD+ products are 4-methyl-5-nitrocatechol + 25% 4-methyl-3-nitrocatechol ?
2,6-dinitrotoluene + NADH + O2
-
 Comamonas sp. 3-methyl-4-nitrocatechol + nitrite + NAD+
-
 ?
naphthalene + NADH + O2
-
 Comamonas sp. cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+ about 60% (-)-cis-1,2-dihydroxy-1,2-dihydronaphthalene ?
nitrobenzene + NADH + O2
-
 Comamonas sp. catechol + nitrite + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
nbzAc
-
 Comamonas sp.