Cloned (Comment) | Organism |
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genes bphAaAbAcAd, encoding the large and small subunits of the terminal oxygenase component and the ferredoxin and reductase subunits responsible for electron transfer from NADH to the large subunit. Functional expression Burkholderia sp. strain NK8 is only successful by combining the bphAaAbAcAd genes of RHA1 and bphA3A4 of Pseudomonas pseudoalcaligenes KF707, introduction of a plasmid containing the RHA1 bphAaAb and KF707 bphA3A4 genes plus the RHA1 bphB2C1D1 genes into strain NK8. The remaining enzyme genes involved in the transformation of biphenyl to benzoate, bphB2C1D1, which encodes dehydrogenase, ring-cleavage dioxygenase and hydrolase, confer activities to NK8, installation of the polychlorinated biphenyls degradation pathway, overview | Rhodococcus jostii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of sets of BphA genes by combining the bphAaAbAcAd genes of RHA1 and bphA3A4 of Pseudomonas pseudoalcaligenes KF707, encoding the ferredoxin and reductase subunits, to obtain the BphA activity of RHA1 in chlorobenzoate-degrading Burkholderia sp. strain NK8, which has an insertion in the cbeA gene to inactivate CBA dioxygenase. Hybrid derivatives of BphA containing the KF707 bphA3 confer BphA activity to NK8, and a derivative containing the RHA1 bphAaAb and KF707 bphA3A4 genes exhibit the highest BphA activity. A plasmid containing the RHA1 bphAaAb and KF707 bphA3A4 genes plus the RHA1 bphB2C1D1 genes is constructed and introduced into strain NK8 | Rhodococcus jostii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl + NADH + H+ + O2 | Rhodococcus jostii | - |
(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl + NADH + H+ + O2 | - |
Rhodococcus jostii | (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BphA | - |
Rhodococcus jostii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodococcus jostii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rhodococcus jostii |
General Information | Comment | Organism |
---|---|---|
additional information | the multicomponent enzyme consists of small and large subunits of the oxygenase component, and ferredoxin and reductase components forming a redox chain in the overall reaction, overview | Rhodococcus jostii |