Cloned (Comment) | Organism |
---|---|
in Escherichia coli, strains M15 and SG13009 | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.094 | - |
biphenyl | parental protein | Comamonas testosteroni | |
1.1 | - |
biphenyl | recombinant His-tagged protein | Comamonas testosteroni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains a [2Fe-2S] Rieske-type center carrying a mononuclear Fe2+ | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25200 | - |
beta subunit with His-tag, SDS-PAGE | Comamonas testosteroni |
44000 | - |
native, recombinant and purified alpha subunit with His-tag, HPLC gel filtration | Comamonas testosteroni |
53600 | - |
alpha subunit with His-tag, SDS-PAGE | Comamonas testosteroni |
186000 | - |
native protein with His-tag, HPLC gel filtration | Comamonas testosteroni |
234000 | - |
native protein, HPLC gel filtration | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Oxidation Stability | Organism |
---|---|
20 min preincubation of His-tagged enzyme with 5 mM dithiothreitol on ice can restore activity of older preparations | Comamonas testosteroni |
Purification (Comment) | Organism |
---|---|
His-tagged ISP, expressed in Escherichia coli | Comamonas testosteroni |
Storage Stability | Organism |
---|---|
-70°C, several months | Comamonas testosteroni |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | alpha3,beta3, 3 * 53600 + 3 * 25200, SDS-PAGE and gel filtration | Comamonas testosteroni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | part of a multicomponent enzyme | Comamonas testosteroni |