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Literature summary for 1.14.12.18 extracted from

  • Hurtubise, Y.; Barriault, D.; Sylvestre, M.
    Characterization of active recombinant His-tagged oxygenase component of Comamonas testosteroni B-356 biphenyl dioxygenase (1996), J. Biol. Chem., 271, 8152-8156.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli, strains M15 and SG13009 Comamonas testosteroni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.094
-
biphenyl parental protein Comamonas testosteroni
1.1
-
biphenyl recombinant His-tagged protein Comamonas testosteroni

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains a [2Fe-2S] Rieske-type center carrying a mononuclear Fe2+ Comamonas testosteroni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25200
-
beta subunit with His-tag, SDS-PAGE Comamonas testosteroni
44000
-
native, recombinant and purified alpha subunit with His-tag, HPLC gel filtration Comamonas testosteroni
53600
-
alpha subunit with His-tag, SDS-PAGE Comamonas testosteroni
186000
-
native protein with His-tag, HPLC gel filtration Comamonas testosteroni
234000
-
native protein, HPLC gel filtration Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
-
-

Oxidation Stability

Oxidation Stability Organism
20 min preincubation of His-tagged enzyme with 5 mM dithiothreitol on ice can restore activity of older preparations Comamonas testosteroni

Purification (Commentary)

Purification (Comment) Organism
His-tagged ISP, expressed in Escherichia coli Comamonas testosteroni

Storage Stability

Storage Stability Organism
-70°C, several months Comamonas testosteroni

Subunits

Subunits Comment Organism
heterohexamer alpha3,beta3, 3 * 53600 + 3 * 25200, SDS-PAGE and gel filtration Comamonas testosteroni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Comamonas testosteroni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
Ferredoxin part of a multicomponent enzyme Comamonas testosteroni