Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 1000 M/s, Km is 0.25 mM | Homo sapiens | |
cytochrome b5 | stimulates NOD activity, reduces the oxidized Cygb-NOD intermediate with a second order rate of 3 x 1000000 M/s, Km is 0.3 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
functional expression of human cytoglobin in rat hepatocytes and effects on the cell NO metabolism, overview. Expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | a competitive inhibitor | Homo sapiens | |
cyanide | causes slow inactivation | Homo sapiens | |
additional information | anions competitively inhibit activity. Increasing the sodium phosphate concentration or including sodium phosphate or NaCl in a 25 mM sodium citrate buffer also competitively inhibits the activity and increases the Km value of ascorbate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten behavior of the Cygb-NOD activity with ascorbate | Homo sapiens | |
0.00002 | - |
O2 | pH 7.0, 37°C | Homo sapiens | |
0.00004 | - |
nitric oxide | pH 7.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatocyte | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 nitric oxide + 2 O2 + NADPH | via an Cygb-NOD intermediate, putative ascorbate-binding site in Cygb, overview | Homo sapiens | 2 nitrate + NADP+ + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cygb | - |
Homo sapiens |
cytoglobin | - |
Homo sapiens |
nitric-oxide dioxygenase | - |
Homo sapiens |
NO dioxygenase | - |
Homo sapiens |
NOD | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is distinct from the microsomal NOD activity | Homo sapiens |