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Literature summary for 1.14.12.15 extracted from

  • Sasoh, M.; Masai, E.; Ishibashi, S.; Hara, H.; Kamimura, N.; Miyauchi, K.; Fukuda, M.
    Characterization of the terephthalate degradation genes of Comamonas sp. strain E6 (2006), Appl. Environ. Microbiol., 72, 1825-1832.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Comamonas sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.5 mM, complete loss of activity Comamonas sp.
EDTA 0.5 mM, complete loss of activity Comamonas testosteroni

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity, inhibited TPADO activity is partially recovered only when Fe2+ is added to the reaction mixture Comamonas testosteroni
Fe2+ required for activity, inhibited TPADO activity is partially recovered only when Fe2+ is added to the reaction mixture Comamonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 subunit TphBII, SDS-PAGE Comamonas sp.

Organism

Organism UniProt Comment Textmining
Comamonas sp.
-
-
-
Comamonas sp. E6
-
-
-
Comamonas testosteroni
-
-
-
Comamonas testosteroni IAM 1152
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 in the presence of 1 mM NADPH, the TPA transformation activity of the crude extract measured in a 1-min reaction is 1.3times higher than that with 1 mM NADH Comamonas testosteroni
additional information
-
 in the presence of 1 mM NADPH, the TPA transformation activity of the crude extract measured in a 1-min reaction is 1.3times higher than that with 1 mM NADH Comamonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas testosteroni ?
-
 ?
additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas sp. ?
-
 ?
additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas testosteroni IAM 1152 ?
-
 ?
additional information phthalate, isophthalate, benzoate, p-hydroxybenzoate, biphenyl, benzene, and toluene are no substrates of TPADO Comamonas sp. E6 ?
-
 ?
terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas testosteroni (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?
terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas sp. (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?
terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas testosteroni IAM 1152 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?
terephthalate + NAD(P)H + O2 + H+ NADPH is the preferred electron donor compared to NADH Comamonas sp. E6 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
-
 ?

Synonyms

Synonyms Comment Organism
TPA 1,2-dioxygenase
-
 Comamonas testosteroni
TPA 1,2-dioxygenase
-
 Comamonas sp.
TPADO TPADO is strongly suggested to be a two-component dioxygenase which consists of the terminal oxygenase component (TphA2 and TphA3) and the reductase component (TphA1) Comamonas testosteroni
TPADO TPADO is strongly suggested to be a two-component dioxygenase which consists of the terminal oxygenase component (TphA2 and TphA3) and the reductase component (TphA1) Comamonas sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Comamonas testosteroni
NADPH
-
 Comamonas sp.