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Literature summary for 1.14.12.14 extracted from

  • Mampel, J.; Ruff, J.; Junker, F.; Cook, A.M.
    The oxygenase component of the 2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1 (1999), Microbiology, 145, 3255-3264.
No PubMed abstract available
Search for more literature for 1.14.12.14

General Stability

General Stability Organism
sensitive to freezing and thawing and exposure to air Alcaligenes sp.

Inhibitors

Inhibitors Comment Organism Structure
o-phenanthroline
-
 Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 benzenesulfonate
-
 Alcaligenes sp.
0.029
-
 2-Aminobenzenesulfonate
-
 Alcaligenes sp.
0.108
-
 4-toluenesulfonate
-
 Alcaligenes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron required Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 alpha-beta, 2 * 46000 + 2 * 16000, SDS-PAGE Alcaligenes sp.
46000
-
 alpha-beta, 2 * 46000 + 2 * 16000, SDS-PAGE Alcaligenes sp.
134000
-
 gel filtration Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminobenzenesulfonate + NADH + O2 Alcaligenes sp. enables utilization of 2-aminobenzenesulfonate as sole carbon and energy source 3-sulfocatechol + NH3 + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzenesulfonate + NADH + O2
-
 Alcaligenes sp. 3-sulfocatechol + NH3 + NAD+
-
 ?
2-aminobenzenesulfonate + NADH + O2 enables utilization of 2-aminobenzenesulfonate as sole carbon and energy source Alcaligenes sp. 3-sulfocatechol + NH3 + NAD+
-
 ?
4-toluenesulfonate + NADH + O2
-
 Alcaligenes sp. ?
-
 ?
benzenesulfonate + NADH + O2
-
 Alcaligenes sp. ?
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha-beta, 2 * 46000 + 2 * 16000, SDS-PAGE Alcaligenes sp.

Synonyms

Synonyms Comment Organism
ABSDOS
-
 Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Alcaligenes sp.

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre one [2Fe-2S] Rieske center per alpha-subunit Alcaligenes sp.
NADH
-
 Alcaligenes sp.