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Literature summary for 1.14.12.13 extracted from

  • Haak, B.; Fetzner, S.; Lingens, F.
    Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS (1995), J. Bacteriol., 177, 667-675.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli TG1, JM105, MV1190 and Pseudomonas putida KT2440 are used as host strains Burkholderia cepacia

Protein Variants

Protein Variants Comment Organism
additional information several mutants of Pseudomonas cepacia, putida and sp. are used Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-sulfur protein Burkholderia cepacia
Sulfide iron-sulfur protein Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19500
-
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE Burkholderia cepacia
37100
-
1 * 37100, NADH:acceptor reductase component, SDS-PAGE Burkholderia cepacia
52400
-
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-chlorobenzoate + NADH + H+ + O2 Burkholderia cepacia initial step in the degradation of aromatic compounds catechol + chloride + NAD+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
2CBS, isolated from water
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange Burkholderia cepacia

Reaction

Reaction Comment Organism Reaction ID
2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 two-component nonheme iron dioxygenase system of oxygenase system class IB dioxygenase: NADH:acceptor reductase containing both FAD and cloroplast-type 2Fe-2S and Rieske-type 2Fe-2S-containing oxygenase component Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-bromobenzoate + NADH + O2
-
Burkholderia cepacia catechol + bromide + NAD+ + CO2
-
?
2-chlorobenzoate + NADH + H+ + O2
-
Burkholderia cepacia catechol + chloride + NAD+ + CO2
-
?
2-chlorobenzoate + NADH + H+ + O2 initial step in the degradation of aromatic compounds Burkholderia cepacia catechol + chloride + NAD+ + CO2
-
?
2-fluorobenzoate + NADH + O2
-
Burkholderia cepacia catechol + fluoride + NAD+ + CO2
-
?
2-iodobenzoate + NADH + O2
-
Burkholderia cepacia catechol + iodide + NAD+ + CO2
-
?
additional information very broad substrate specificity Burkholderia cepacia ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37100, NADH:acceptor reductase component, SDS-PAGE Burkholderia cepacia
oligomer x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
FAD
-
Burkholderia cepacia
NADH
-
Burkholderia cepacia