Cloned (Comment) | Organism |
---|---|
Escherichia coli TG1, JM105, MV1190 and Pseudomonas putida KT2440 are used as host strains | Burkholderia cepacia |
Protein Variants | Comment | Organism |
---|---|---|
additional information | several mutants of Pseudomonas cepacia, putida and sp. are used | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | iron-sulfur protein | Burkholderia cepacia | |
Sulfide | iron-sulfur protein | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19500 | - |
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE | Burkholderia cepacia |
37100 | - |
1 * 37100, NADH:acceptor reductase component, SDS-PAGE | Burkholderia cepacia |
52400 | - |
x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 | Burkholderia cepacia | initial step in the degradation of aromatic compounds | catechol + chloride + NAD+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
2CBS, isolated from water | - |
Purification (Comment) | Organism |
---|---|
anion-exchange | Burkholderia cepacia |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 | two-component nonheme iron dioxygenase system of oxygenase system class IB dioxygenase: NADH:acceptor reductase containing both FAD and cloroplast-type 2Fe-2S and Rieske-type 2Fe-2S-containing oxygenase component | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-bromobenzoate + NADH + O2 | - |
Burkholderia cepacia | catechol + bromide + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | - |
Burkholderia cepacia | catechol + chloride + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | initial step in the degradation of aromatic compounds | Burkholderia cepacia | catechol + chloride + NAD+ + CO2 | - |
? | |
2-fluorobenzoate + NADH + O2 | - |
Burkholderia cepacia | catechol + fluoride + NAD+ + CO2 | - |
? | |
2-iodobenzoate + NADH + O2 | - |
Burkholderia cepacia | catechol + iodide + NAD+ + CO2 | - |
? | |
additional information | very broad substrate specificity | Burkholderia cepacia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37100, NADH:acceptor reductase component, SDS-PAGE | Burkholderia cepacia |
oligomer | x * 19500, x * 52400, alpha3beta3, small and large subunit of the oxygenase component, SDS-PAGE | Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Burkholderia cepacia | |
NADH | - |
Burkholderia cepacia |