Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants are made by treatment with N-methyl-N'-nitro-N-nitrosoguanidine, mutants are unable to grow on 2-chlorobenzoate and catechol | Burkholderia cepacia |
General Stability | Organism |
---|---|
sucrose has stabilizing effect | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | exogenous, required for activity | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 | Burkholderia cepacia | first step of 2-chlorobenzoate degradation via meta-cleavage | catechol + chloride + NAD+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
2CBS, isolated from water | - |
Purification (Comment) | Organism |
---|---|
anion exchange | Burkholderia cepacia |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 | multi-component dioxygenase system proposed: 1,2-hydroxylating, dehalogenating, decarboxylating | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0085 | - |
crude extract, 3.4 mg protein per ml in the assay | Burkholderia cepacia |
0.043 | - |
after anion exchange: fraction A + B + NADH + Fe2+ | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chlorobenzoate + NADH + H+ + O2 | the oxygen in the enzymatically catechol is derived from molecular oxygen, the formed byproduct 2,3-dihydroxybenzoate is suggested to be a dead end metabolite | Burkholderia cepacia | catechol + chloride + NAD+ + CO2 | - |
? | |
2-chlorobenzoate + NADH + H+ + O2 | first step of 2-chlorobenzoate degradation via meta-cleavage | Burkholderia cepacia | catechol + chloride + NAD+ + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
assay at | Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | can not be replaced by NADPH | Burkholderia cepacia |