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Literature summary for 1.14.12.11 extracted from

  • Vila, M.; Umpierrez, D.; Veiga, N.; Seoane, G.; Carrera, I.; Rodriguez Giordano, S.
    Site-directed mutagenesis studies on the toluene dioxygenase enzymatic system Role of phenylalanine 366, threonine 365 and isoleucine 324 in the chemo-, regio-, and stereoselectivity (2017), Adv. Synth. Catal., 359, 2149-2157 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
F366 the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas putida
I324F the mutant shows reduced activity compared to the wild type enzyme Pseudomonas putida
T365N the mutant shows slightly reduced activity compared to the wild type enzyme Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-dinitrobenzene + NADH + H+ + O2
-
Pseudomonas putida ?
-
?
bromobenzene + NADH + H+ + O2
-
Pseudomonas putida cis-(1S,2S)-3-bromocyclohexa-3,5-diene-1,2-diol + NAD+
-
?
indene + NADH + H+ + O2
-
Pseudomonas putida cis-(1S,2R)-indandiol + (1S)-indenol + NAD+
-
?
propylbenzene + NADH + H+ + O2
-
Pseudomonas putida cis-(1S,2R)-3-propylcyclohexa-3,5-diene-1,2-diol + cis-(1S,2R)-3-[(1R)-1-hydroxypropyl]cyclohexa-3,5-diene-1,2-diol + NAD+
-
?
styrene + NADH + H+ + O2
-
Pseudomonas putida cis-2,3-dihydroxy-1-vinylcyclohexa-4,6-diene + (R)1-phenyl-1,2-ethanediol + NAD+
-
?
toluene + NADH + H+ + O2
-
Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Synonyms

Synonyms Comment Organism
TDO
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida