Literature summary for 1.14.12.11 extracted from

Lacal, J.; Guazzaroni, M.E.; Busch, A.; Krell, T.; Ramos, J.L.
Hierarchical binding of the TodT response regulator to its multiple recognition sites at the tod pathway operon promoter (2008), J. Mol. Biol., 376, 325-337.

Search for more literature for 1.14.12.11

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type TodT, C-terminal part of TodT, and TodT mutants in Escherichia coli strain BL21(DE3). Genes todS and todT are transcribed from a single promoter called PtodX once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	mutations in the different TodT boxes affect TodT binding to the mutant promoters, overview	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	thermodynamic characterization of the molecular interactions between TodT and the C-TodT protein with individual TodT boxes	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	phosphorylated TodT, gel filtration	Pseudomonas putida
108000	-	TodS	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Pseudomonas putida	TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated as an increase in the rate of transphosphorylation of TodT	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	genes todS and todT, the expression of the catabolic todXFC1C2BADEGIH operon takes place from a single promoter termed PtodX, located upstream from the todX gene. Its activation is mediated by the TodS/TodT TCS in response to toluene and other aromatic hydrocarbons. The todST genes, located downstream from todH, form an independent operon that is expressed constitutively	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated as an increase in the rate of transphosphorylation of TodT, TodT phosphorylation occurs only with the folded protein by the TodS sensor kinase, binding site analysis, overview	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated as an increase in the rate of transphosphorylation of TodT	Pseudomonas putida	?	-	?
additional information	TodT-P is the active form of the response regulator that induces RNA polymerase to transcribe from the PtodX promoter	Pseudomonas putida	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 x * 28000, TodT is a monomer in solution	Pseudomonas putida
More	in TodT, the C-terminal domain has about 65 amino acids and contains a helix-turn-helix domain	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
Tod	-	Pseudomonas putida

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pseudomonas putida	about, TodT	-	7.8

General Information

General Information	Comment	Organism
metabolism	TodS and TodT proteins form a highly specific two-component regulatory system that controls the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)