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Literature summary for 1.14.12.11 extracted from

  • Subramanian, V.; Liu, T.N.; Yeh, W.K.; Serdar, C.M.; Wackett, L.P.; Gibson, D.T.
    Purification and properties of ferredoxinTOL. A component of toluene dioxygenase from Pseudomonas putida F1 (1985), J. Biol. Chem., 260, 2355-2363.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron ferredoxinTOL component of the toluene dioxygenase contains 2 gatoms each of iron and acid-labile sulfur which appear to be organized as a single [2Fe-2S]cluster Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14700
-
ferredoxinTOL component of the toluene dioxygenase, equilibrium sedimentation Pseudomonas putida
15500
-
1 * 15500, ferredoxinTOL component of the toluene dioxygenase, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ferredoxinTOL component of the toluene dioxygenase Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, ferredoxinTOL component of the toluene dioxygenase, stable for over 10 weeks Pseudomonas putida
0-4°C, ferredoxinTOL component of the toluene dioxygenase, stable for up to 72 h Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
toluene + NADH + O2
-
Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 15500, ferredoxinTOL component of the toluene dioxygenase, SDS-PAGE Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida