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Literature summary for 1.14.12.11 extracted from

  • Subramanian, V.; Liu, T.N.; Yeh, W.K.; Gibson, D.T.
    Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography (1979), Biochem. Biophys. Res. Commun., 91, 1131-1139.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Pseudomonas putida
Iron iron-sulfur protein contains 2 gatom of iron and 2 gatom of acid-labile sulfur per mol of protein Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the enzyme system consists of monomeric reductaseTOL and ferredoxinTOL and a dimeric terminal oxygenase Pseudomonas putida
20800
-
x * 52500 + x * 20800, oxygenase component, SDS-PAGE Pseudomonas putida
52500
-
x * 52500 + x * 20800, oxygenase component, SDS-PAGE Pseudomonas putida
151000
-
oxygenase component, gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
oxygenase component, ISPTOL Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0075
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
toluene + NADH + O2
-
Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 52500 + x * 20800, oxygenase component, SDS-PAGE Pseudomonas putida
More the enzyme system consists of monomeric reductaseTOL, a ferredoxinTOL and a dimeric terminal oxygenase Pseudomonas putida

Synonyms

Synonyms Comment Organism
ISPTOL oxygenase component of the toluene dioxygenase multienzyme system Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida