Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Pseudomonas putida | |
Iron | iron-sulfur protein contains 2 gatom of iron and 2 gatom of acid-labile sulfur per mol of protein | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme system consists of monomeric reductaseTOL and ferredoxinTOL and a dimeric terminal oxygenase | Pseudomonas putida |
20800 | - |
x * 52500 + x * 20800, oxygenase component, SDS-PAGE | Pseudomonas putida |
52500 | - |
x * 52500 + x * 20800, oxygenase component, SDS-PAGE | Pseudomonas putida |
151000 | - |
oxygenase component, gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
oxygenase component, ISPTOL | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0075 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + NADH + O2 | - |
Pseudomonas putida | (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52500 + x * 20800, oxygenase component, SDS-PAGE | Pseudomonas putida |
More | the enzyme system consists of monomeric reductaseTOL, a ferredoxinTOL and a dimeric terminal oxygenase | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
ISPTOL | oxygenase component of the toluene dioxygenase multienzyme system | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas putida |