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Literature summary for 1.14.12.10 extracted from

  • Rivard, B.S.; Rogers, M.S.; Marell, D.J.; Neibergall, M.B.; Chakrabarty, S.; Cramer, C.J.; Lipscomb, J.D.
    Rate-determining attack on substrate precedes rieske cluster oxidation during cis-dihydroxylation by benzoate dioxygenase (2015), Biochemistry, 54, 4652-4664 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoate + NADH + H+ + O2 Pseudomonas putida
-
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
?
benzoate + NADH + H+ + O2 Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoate + NADH + H+ + O2
-
Pseudomonas putida (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
?
benzoate + NADH + H+ + O2
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
-
?

Synonyms

Synonyms Comment Organism
benzoate 1,2-dioxygenase oxygenase
-
Pseudomonas putida
BZDO
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
benzoate at pH 6.8 temperature not specified in the publication Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas putida
[2Fe-2S]-center
-
Pseudomonas putida