Cloned (Comment) | Organism |
---|---|
expressed in Pseudomonas putida CL01 as His-tag fusion protein | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
benzoate | pH 7, 25°C | Acinetobacter calcoaceticus | |
0.089 | - |
3-methylbenzoate | pH 7, 25°C | Acinetobacter calcoaceticus | |
0.113 | - |
3-chlorobenzoate | pH 7, 25°C | Acinetobacter calcoaceticus | |
1.401 | - |
2-Methylbenzoate | pH 7, 25°C | Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | active site mononuclear iron | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoate + O2 + NADH | Acinetobacter calcoaceticus | catabolism of aromatic compounds | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
benzoate + O2 + NADH | Acinetobacter calcoaceticus BADO ADP1 | catabolism of aromatic compounds | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Acinetobacter calcoaceticus BADO ADP1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant and native enzyme | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
pH 7, 25°C, with 3-methylbenzoate as substrate | Acinetobacter calcoaceticus |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 6 months | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbenzoate + O2 + NADH | - |
Acinetobacter calcoaceticus | cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+ | - |
? | |
2-methylbenzoate + O2 + NADH | - |
Acinetobacter calcoaceticus BADO ADP1 | cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+ | - |
? | |
3-chlorobenzoate + O2 + NADH + H+ | - |
Acinetobacter calcoaceticus | 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-chlorobenzoate + O2 + NADH + H+ | - |
Acinetobacter calcoaceticus BADO ADP1 | 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-methylbenzoate + O2 + NADH + H+ | - |
Acinetobacter calcoaceticus | 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-methylbenzoate + O2 + NADH + H+ | - |
Acinetobacter calcoaceticus BADO ADP1 | 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
benzoate + O2 + NADH | - |
Acinetobacter calcoaceticus | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
benzoate + O2 + NADH | catabolism of aromatic compounds | Acinetobacter calcoaceticus | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
benzoate + O2 + NADH | - |
Acinetobacter calcoaceticus BADO ADP1 | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
benzoate + O2 + NADH | catabolism of aromatic compounds | Acinetobacter calcoaceticus BADO ADP1 | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
3-chlorobenzoate | pH 7, 25°C | Acinetobacter calcoaceticus | |
4.2 | - |
2-Methylbenzoate | pH 7, 25°C | Acinetobacter calcoaceticus | |
7 | - |
3-methylbenzoate | pH 7, 25°C | Acinetobacter calcoaceticus | |
8.6 | - |
benzoate | pH 7, 25°C | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | [2Fe-2S] Rieske center | Acinetobacter calcoaceticus | |
NADH | - |
Acinetobacter calcoaceticus |