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Literature summary for 1.14.12.10 extracted from

  • Ge, Y.; Eltis, L.D.
    Characterization of hybrid toluate and benzoate dioxygenases (2003), J. Bacteriol., 185, 5333-5341.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pseudomonas putida CL01 as His-tag fusion protein Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
benzoate pH 7, 25°C Acinetobacter calcoaceticus
0.089
-
3-methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus
0.113
-
3-chlorobenzoate pH 7, 25°C Acinetobacter calcoaceticus
1.401
-
2-Methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron active site mononuclear iron Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoate + O2 + NADH Acinetobacter calcoaceticus catabolism of aromatic compounds 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH Acinetobacter calcoaceticus BADO ADP1 catabolism of aromatic compounds 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-
Acinetobacter calcoaceticus BADO ADP1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant and native enzyme Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
pH 7, 25°C, with 3-methylbenzoate as substrate Acinetobacter calcoaceticus

Storage Stability

Storage Stability Organism
-80°C, stable for at least 6 months Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylbenzoate + O2 + NADH
-
Acinetobacter calcoaceticus cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
?
2-methylbenzoate + O2 + NADH
-
Acinetobacter calcoaceticus BADO ADP1 cis-1,2-dihydro-1,2-dihydroxy-2-methyl-benzoate + NAD+
-
?
3-chlorobenzoate + O2 + NADH + H+
-
Acinetobacter calcoaceticus 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
3-chlorobenzoate + O2 + NADH + H+
-
Acinetobacter calcoaceticus BADO ADP1 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
3-methylbenzoate + O2 + NADH + H+
-
Acinetobacter calcoaceticus 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
3-methylbenzoate + O2 + NADH + H+
-
Acinetobacter calcoaceticus BADO ADP1 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH
-
Acinetobacter calcoaceticus 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH catabolism of aromatic compounds Acinetobacter calcoaceticus 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH
-
Acinetobacter calcoaceticus BADO ADP1 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH catabolism of aromatic compounds Acinetobacter calcoaceticus BADO ADP1 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
3-chlorobenzoate pH 7, 25°C Acinetobacter calcoaceticus
4.2
-
2-Methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus
7
-
3-methylbenzoate pH 7, 25°C Acinetobacter calcoaceticus
8.6
-
benzoate pH 7, 25°C Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre [2Fe-2S] Rieske center Acinetobacter calcoaceticus
NADH
-
Acinetobacter calcoaceticus