Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | oxygenase component: 2.7 mol/mol of alphabeta subunit, each alpha subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site, reductase component: 2.02 mol/monomer | Pseudomonas putida | |
Iron | each alpha-subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
alpha3,beta3, 3 * 19000 + 3 * 49000, oxygenase component, SDS-PAGE | Pseudomonas putida |
38000 | - |
reductase component | Pseudomonas putida |
49000 | - |
alpha3,beta3, 3 * 19000 + 3 * 49000, oxygenase component, SDS-PAGE | Pseudomonas putida |
195000 | - |
sedimentation analysis | Pseudomonas putida |
195000 | - |
oxygenase component, sedimentation analysis | Pseudomonas putida |
204000 | - |
SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
benzoate oxygenase and reductase component | Pseudomonas putida |
DEAE-Sepharose Fast Flow column chromatography and Ultrogel AcA 34 gel filtration | Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ | proposed catalytic cycle | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.36 | - |
benzoate oxygenase | Pseudomonas putida |
790 | - |
benzoate reductase, reduction of K3Fe(CN)6 | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chlorobenzoate + O2 + NADH + H+ | - |
Pseudomonas putida | 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-chlorobenzoate + O2 + NADH + H+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 3-chloro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-fluorobenzoate + O2 + NADH + H+ | - |
Pseudomonas putida | 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-fluorobenzoate + O2 + NADH + H+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 3-fluoro-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-methylbenzoate + O2 + NADH + H+ | - |
Pseudomonas putida | 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
3-methylbenzoate + O2 + NADH + H+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 3-methyl-2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
benzoate + O2 + NADH | - |
Pseudomonas putida | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? | |
benzoate + O2 + NADH | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 2-hydro-1,2-dihydroxybenzoate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha3,beta3, 3 * 19000 + 3 * 49000, oxygenase component, SDS-PAGE | Pseudomonas putida |
monomer | 1 * 38000, reductase component, sedimentation analysis, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
BZDO | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 FAD per enzyme monomer | Pseudomonas putida | |
iron-sulfur center | each alpha-subunit contains a Rieske [2Fe-2S] cluster and a mononuclear iron site | Pseudomonas putida | |
NADH | - |
Pseudomonas putida |