BRENDA - Enzyme Database
show all sequences of 1.14.11.B18

Allelic variants from Dahlia variabilis encode with functional differences in chalcone 3-hydroxylase activity

Schlangen, K.; Miosic, S.; Halbwirth, H.; Arch. Biochem. Biophys. 494, 40-45 (2010)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Dahlia pinnata
Protein Variants
Protein Variants
Commentary
Organism
V425A
complete loss of the ability th hydroxylate isoliquiritigenin in position 3
Dahlia pinnata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
isoliquiritigenin
pH 7.5, 30C
Dahlia pinnata
Organism
Organism
UniProt
Commentary
Textmining
Dahlia pinnata
D3IWE6
D3IWE6 i.e. allele 2, enzyme additionally catalyzes the reaction of EC 1.14.14.82
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
isoliquiritigenin + 2-oxoglutarate + O2
hydroxylation in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
butein + succinate + CO2
-
-
-
?
naringenin chalcone + 2-oxoglutarate + O2
-
744156
Dahlia pinnata
eriodictyol chalcone
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Cyp75B
-
Dahlia pinnata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Dahlia pinnata
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Dahlia pinnata
Engineering (protein specific)
Protein Variants
Commentary
Organism
V425A
complete loss of the ability th hydroxylate isoliquiritigenin in position 3
Dahlia pinnata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
isoliquiritigenin
pH 7.5, 30C
Dahlia pinnata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
isoliquiritigenin + 2-oxoglutarate + O2
hydroxylation in position 3, i.e. reaction of chalcone 3-hydroxylase
744156
Dahlia pinnata
butein + succinate + CO2
-
-
-
?
naringenin chalcone + 2-oxoglutarate + O2
-
744156
Dahlia pinnata
eriodictyol chalcone
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Dahlia pinnata
Other publictions for EC 1.14.11.B18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
744156
Schlangen
Allelic variants from Dahlia ...
Dahlia pinnata
Arch. Biochem. Biophys.
494
40-45
2010
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