Literature summary for 1.14.11.9 extracted from

Li, C.; Liu, S.; Yao, X.; Wang, J.; Wang, T.; Zhang, Z.; Zhang, P.; Chen, K.
PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis (2017), Plant Growth Regul., 83, 489-500 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbate	required	Pohlia nutans

Cloned(Commentary)

Cloned (Comment)	Organism
gene PnF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Arabidopsis thaliana ecotype Col-0 via Agrobacterium tumefaciens-mediated transformation conferring tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana	Pohlia nutans

Cloned (Comment)

Organism

gene PnF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Arabidopsis thaliana ecotype Col-0 via Agrobacterium tumefaciens-mediated transformation conferring tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana

Pohlia nutans

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of transgenic Arabidospis thaliana plants expressing the enzyme from Pohlia nutans, the Agrobacterium tumefaciens-mediated transformation of gene PnF3H confers tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, naringenin-feeding experiments and flavonoid metabolism profiling by HPLC analysis, phenotype, overview	Pohlia nutans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	required for activity	Pohlia nutans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2	Pohlia nutans	-	a (2R,3R)-dihydroflavonol + succinate + CO2	-	?
naringenin + 2-oxoglutarate + O2	Pohlia nutans	-	dihydrokaempferol + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pohlia nutans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2	-	Pohlia nutans	a (2R,3R)-dihydroflavonol + succinate + CO2	-	?
naringenin + 2-oxoglutarate + O2	-	Pohlia nutans	dihydrokaempferol + succinate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
F3H	-	Pohlia nutans
flavanone 3-hydroxylase	-	Pohlia nutans
PnF3H	-	Pohlia nutans

Expression

Organism	Comment	Expression
Pohlia nutans	PnF3H expression levels increase by 2.49fold at 1 h and then slightly decrease to 1.47fold at 24 h with cold treatment. For the simulated drought stress (i.e. 20% PEG 6000), the PnF3H expression levels enhance by 2.39fold at 3 h and the transcript abundance remains high up to 12 h. In the UV-B radiation, the PnF3H expression levels increase and reach the maximum level of 4.57fold at 3 h. After exogenous abscisic acid treatment, the PnF3H expression levels increase quickly and reach the maximum level of 2.57fold at 0.5 h	up

General Information

General Information	Comment	Organism
physiological function	the enzyme plays a role in plant salt stress resistance. PnF3H transcripts are induced by various stress and may play a positive role in stress tolerance	Pohlia nutans