Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.9 extracted from

  • Khumkarjorn, N.; Thanonkeo, S.; Yamada, M.; Thanonkeo, P.
    Cloning and expression analysis of a flavanone 3-hydroxylase gene in Ascocenda orchid (2017), J. Plant Biochem. Biotechnol., 26, 179-190 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ascorbate required x Ascocenda

Cloned(Commentary)

Cloned (Comment) Organism
gene F3H cloned from petals, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21 x Ascocenda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.207
-
naringenin pH 8.0, 45°C x Ascocenda

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalytic activity x Ascocenda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2 x Ascocenda
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2 x Ascocenda NK-2012
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
?
naringenin + 2-oxoglutarate + O2 x Ascocenda
-
dihydrokaempferol + succinate + CO2
-
?
naringenin + 2-oxoglutarate + O2 x Ascocenda NK-2012
-
dihydrokaempferol + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
x Ascocenda J9QYL0 var. Subun
-
x Ascocenda NK-2012 J9QYL0 var. Subun
-

Source Tissue

Source Tissue Comment Organism Textmining
flower enzyme expression analysis of AcF3H at different developmental stages and in different parts of the flower, overview. The highest enzyme expression occurs when the petals turned pale-orange x Ascocenda
-
petal
-
x Ascocenda
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
x Ascocenda a (2R,3R)-dihydroflavonol + succinate + CO2
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
x Ascocenda NK-2012 a (2R,3R)-dihydroflavonol + succinate + CO2
-
?
naringenin + 2-oxoglutarate + O2
-
x Ascocenda dihydrokaempferol + succinate + CO2
-
?
naringenin + 2-oxoglutarate + O2
-
x Ascocenda NK-2012 dihydrokaempferol + succinate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 41220, sequence calculation, x * 41500, recombinant enzyme, SDS-PAGE x Ascocenda

Synonyms

Synonyms Comment Organism
AcF3H
-
x Ascocenda
F3H
-
x Ascocenda
flavanone 3-hydroxylase
-
x Ascocenda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
recombinant enzyme x Ascocenda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 recombinant enzyme, stable at 20-45°C, loss of activity above 45°C, inactivation at 60°C x Ascocenda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant enzyme x Ascocenda

pI Value

Organism Comment pI Value Maximum pI Value
x Ascocenda sequence calculation
-
5.1