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Literature summary for 1.14.11.9 extracted from

  • Kallscheuer, N.; Vogt, M.; Bott, M.; Marienhagen, J.
    Functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin (2017), J. Biotechnol., 258, 190-196 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin Petunia x hybrida
synthesis expression of heterologous dioxygenase genes in (2S)-flavanone-producing Corynebacterium glutamicum strains enables the production of flavanonols and flavonols, e.g kaempferol and quercetin, starting from thephenylpropanoids p-coumaric acid and caffeic acid Petunia x hybrida

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin Petunia x hybrida
gene F3H, functional recombinant expression of codon-optimized enzyme in Corynebacterium glutamicum strain DelAro4, coexpression with gene FLS encoding flavonol synthase from Populus deltoides. Coexpression fo genes F3H and FLS both from Petunia hybrida is not successful Petunia x hybrida

Protein Variants

Protein Variants Comment Organism
additional information the enzyme is ngineered for synthesizing polyphenol core structuressuch as the stilbene resveratrol and the (2S)-flavanone naringenin Petunia x hybrida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2 Petunia x hybrida
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Petunia x hybrida O22530
-
-
Petunia x hybrida Q07512
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Petunia x hybrida a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
eriodictyol + 2-oxoglutarate + O2
-
 Petunia x hybrida dihydroquercetin + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Petunia x hybrida dihydrokaempferol + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
F3H
-
 Petunia x hybrida
flavanone 3-hydroxylase
-
 Petunia x hybrida

General Information

General Information Comment Organism
evolution the enzyme belongs to the family of 2-oxoglutarate-dependent dioxygenases Petunia x hybrida
metabolism (2S)-flavanones are converted to flavonols by the activity of the 2-oxoglutarate-dependent dioxygenases flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) Petunia x hybrida