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Literature summary for 1.14.11.9 extracted from

  • Halbwirth, H.; Waldner, I.; Miosic, S.; Ibanez, M.; Costa, G.; Stich, K.
    Measuring flavonoid enzyme activities in tissues of fruit species (2009), J. Agric. Food Chem., 57, 4983-4987.
    View publication on PubMed
Search for more literature for 1.14.11.9

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Prunus avium
Fe2+
-
 Rubus plicatus
Fe2+
-
 Prunus domestica
Fe2+
-
 Rubus idaeus
Fe2+
-
 Sambucus nigra
Fe2+
-
 Prunus cerasus
Fe2+
-
 Ribes uva-crispa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Prunus avium two different protocols for enzyme preparation from eight fruit species ?
-
 ?
additional information Rubus plicatus two different protocols for enzyme preparation from eight fruit species ?
-
 ?
additional information Prunus domestica two different protocols for enzyme preparation from eight fruit species ?
-
 ?
additional information Rubus idaeus two different protocols for enzyme preparation from eight fruit species ?
-
 ?
additional information Sambucus nigra two different protocols for enzyme preparation from eight fruit species ?
-
 ?
additional information Prunus cerasus two different protocols for enzyme preparation from eight fruit species ?
-
 ?
additional information Ribes uva-crispa two different protocols for enzyme preparation from eight fruit species ?
-
 ?
naringenin + O2 + 2-oxoglutarate Prunus avium 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate Rubus plicatus 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate Prunus domestica 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate Rubus idaeus 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate Sambucus nigra 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate Prunus cerasus 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate Ribes uva-crispa 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) dihydrokaempferol + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
no activity in Actinidia deliciosa
-
 green-fleshed cultivar Hayward
-
no activity in Actinidia deliciosa
-
 kiwi fruit, cultivar Hayward, ripe fruit and leaves
-
Prunus avium
-
 cultivar Augustkirsche, cherry
-
Prunus avium
-
 cv. Augustkirsche
-
Prunus cerasus
-
 cultivar Pandy 114, sour cherry
-
Prunus cerasus
-
 cv. Pandy 114
-
Prunus domestica
-
-
-
Prunus domestica
-
 plum
-
Ribes uva-crispa
-
-
-
Ribes uva-crispa
-
 gooseberry
-
Rubus idaeus
-
-
-
Rubus idaeus
-
 raspberry
-
Rubus plicatus
-
-
-
Rubus plicatus
-
 blackberry
-
Sambucus nigra
-
-
-
Sambucus nigra
-
 elder
-

Purification (Commentary)

Purification (Comment) Organism
shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30 mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column Prunus avium
shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30 mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column Rubus plicatus
shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30 mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column Prunus domestica
shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30 mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column Rubus idaeus
shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30 mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column Prunus cerasus
shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30 mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column Ribes uva-crispa
shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column Sambucus nigra

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Sambucus nigra
-
fruit
-
 Prunus avium
-
fruit
-
 Rubus plicatus
-
fruit
-
 Sambucus nigra
-
fruit
-
 Prunus cerasus
-
fruit no activity in ripe fruits Prunus domestica
-
fruit no activity in ripe fruits Ribes uva-crispa
-
fruit no activity in unripe fruits Rubus idaeus
-
fruit ripe and unripe (50% size, uncolored) Rubus plicatus
-
fruit ripe and unripe (50% size, uncolored) Prunus domestica
-
fruit ripe and unripe (50% size, uncolored) Rubus idaeus
-
fruit ripe and unripe (50% size, uncolored) Sambucus nigra
-
fruit ripe and unripe (50% size, uncolored) Prunus cerasus
-
fruit ripe and unripe (50% size, uncolored) Ribes uva-crispa
-
fruit ripe fruit Prunus avium
-
leaf
-
 Prunus avium
-
leaf
-
 Rubus plicatus
-
leaf
-
 Prunus domestica
-
leaf
-
 Rubus idaeus
-
leaf
-
 Sambucus nigra
-
leaf
-
 Ribes uva-crispa
-
leaf young, enrolling leaves Prunus avium
-
leaf young, enrolling leaves Rubus plicatus
-
leaf young, enrolling leaves Prunus domestica
-
leaf young, enrolling leaves Rubus idaeus
-
leaf young, enrolling leaves Sambucus nigra
-
leaf young, enrolling leaves Prunus cerasus
-
leaf young, enrolling leaves Ribes uva-crispa
-
additional information no activity in leaves, protein content of the enzyme in the different plants and tissue preparations obtained with two different protocols Prunus cerasus
-
additional information protein content of the enzyme in the different plants and tissue preparations obtained with two different protocols Prunus avium
-
additional information protein content of the enzyme in the different plants and tissue preparations obtained with two different protocols Rubus plicatus
-
additional information protein content of the enzyme in the different plants and tissue preparations obtained with two different protocols Prunus domestica
-
additional information protein content of the enzyme in the different plants and tissue preparations obtained with two different protocols Rubus idaeus
-
additional information protein content of the enzyme in the different plants and tissue preparations obtained with two different protocols Sambucus nigra
-
additional information protein content of the enzyme in the different plants and tissue preparations obtained with two different protocols Ribes uva-crispa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no activity in leaves, whether with enzyme extraction protocol 1 or 2 Prunus cerasus
additional information
-
 no activity in ripe fruit, whether with enzyme extraction protocol 1 or 2 Prunus domestica
additional information
-
 no activity in unripe fruit, whether with enzyme extraction protocol 1 or 2 Rubus idaeus
0.0000026
-
 unripe fruit: 43 nkat/kg total protein (enzyme extraction protocol 2) Sambucus nigra
0.0000038
-
 flower: 64 nkat/kg total protein (enzyme extraction protocol 1) Sambucus nigra
0.0000079
-
 ripe fruit: 131 nkat/kg total protein (enzyme extraction protocol 2), no activity with enzyme extraction protocol 1 Ribes uva-crispa
0.000012
-
 leaves: 198 nkat/kg total protein (enzyme extraction protocol 1) Rubus plicatus
0.000012
-
 leaves: 198 nkat/kg total protein (enzyme extraction protocol 2) Rubus plicatus
0.000012
-
 unripe fruit: 196 nkat/kg total protein (enzyme extraction protocol 1) Ribes uva-crispa
0.000015
-
 unripe fruit: 242 nkat/kg total protein (enzyme extraction protocol 1) Sambucus nigra
0.000019
-
 leaves: 316 nkat/kg total protein (enzyme extraction protocol 1), no activity with enzyme extraction protocol 2 Prunus domestica
0.000021
-
 ripe fruit: 357 nkat/kg total protein (enzyme extraction protocol 2) Prunus avium
0.000023
-
 ripe fruit: 388 nkat/kg total protein (enzyme extraction protocol 1) Rubus plicatus
0.000026
-
 unripe fruit: 440 nkat/kg total protein (enzyme extraction protocol 2), no activity with enzyme extraction protocol 1 Rubus plicatus
0.000027
-
 ripe fruit: 443 nkat/kg total protein (enzyme extraction protocol 2) Rubus plicatus
0.000028
-
 flower: 46 nkat/kg total protein (enzyme extraction protocol 2) Sambucus nigra
0.000029
-
 ripe fruit: 484 nkat/kg total protein (enzyme extraction protocol 2) Sambucus nigra
0.000036
-
 leaves: 606 nkat/kg total protein (enzyme extraction protocol 2) Prunus avium
0.000041
-
 leaves: 690 nkat/kg total protein (enzyme extraction protocol 1), no activity with enzyme extraction protocol 2 Sambucus nigra
0.000041
-
 ripe fruit: 688 nkat/kg total protein (enzyme extraction protocol 1) Sambucus nigra
0.000045
-
 leaves: 758 nkat/kg total protein (enzyme extraction protocol 1) Prunus avium
0.00005
-
 unripe fruit: 833 nkat/kg total protein (enzyme extraction protocol 2), no activity with enzyme extraction protocol 1 Prunus domestica
0.000056
-
 ripe fruit: 932 nkat/kg total protein (enzyme extraction protocol 1) Prunus avium
0.000062
-
 unripe fruit: 1039 nkat/kg total protein (enzyme extraction protocol 2) Ribes uva-crispa
0.000066
-
 leaves: 1105 nkat/kg total protein (enzyme extraction protocol 2), no activity with enzyme extraction protocol 1 Rubus idaeus
0.000092
-
 ripe fruit: 1537 nkat/kg total protein (enzyme extraction protocol 2), no activity with enzyme extraction protocol 1 Rubus idaeus
0.00012
-
 leaves: 1933 nkat/kg total protein (enzyme extraction protocol 2), no activity with enzyme extraction protocol 1 Ribes uva-crispa
0.00026
-
 unripe fruit: 4400 nkat/kg total protein (enzyme extraction protocol 2), no activity with enzyme extraction protocol 1 Prunus cerasus
0.0009
-
 ripe fruit: 15072 nkat/kg total protein (enzyme extraction protocol 1) Prunus cerasus
0.00094
-
 ripe fruit: 15652 nkat/kg total protein (enzyme extraction protocol 2) Prunus cerasus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information two different protocols for enzyme preparation from eight fruit species Prunus avium ?
-
 ?
additional information two different protocols for enzyme preparation from eight fruit species Rubus plicatus ?
-
 ?
additional information two different protocols for enzyme preparation from eight fruit species Prunus domestica ?
-
 ?
additional information two different protocols for enzyme preparation from eight fruit species Rubus idaeus ?
-
 ?
additional information two different protocols for enzyme preparation from eight fruit species Sambucus nigra ?
-
 ?
additional information two different protocols for enzyme preparation from eight fruit species Prunus cerasus ?
-
 ?
additional information two different protocols for enzyme preparation from eight fruit species Ribes uva-crispa ?
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Prunus avium (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Rubus plicatus (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Prunus domestica (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Rubus idaeus (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Sambucus nigra (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Prunus cerasus (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Ribes uva-crispa (2R,3R)-dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) Prunus avium dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) Rubus plicatus dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) Prunus domestica dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) Rubus idaeus dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) Sambucus nigra dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) Prunus cerasus dihydrokaempferol + succinate + CO2
-
 ?
naringenin + O2 + 2-oxoglutarate 0.046 nmol 14C-labeled substrate, 3.48 mM 2-oxoglutarate, 5 mM FeSO4 * 7 H2O, buffer (0.1 M Tris/HCl, pH 7.5, containing 0.4% sodium ascorbate) Ribes uva-crispa dihydrokaempferol + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
FHT
-
 Prunus avium
FHT
-
 Rubus plicatus
FHT
-
 Prunus domestica
FHT
-
 Rubus idaeus
FHT
-
 Sambucus nigra
FHT
-
 Prunus cerasus
FHT
-
 Ribes uva-crispa
flavanone 3-dioxygenase
-
 Prunus avium
flavanone 3-dioxygenase
-
 Rubus plicatus
flavanone 3-dioxygenase
-
 Prunus domestica
flavanone 3-dioxygenase
-
 Rubus idaeus
flavanone 3-dioxygenase
-
 Sambucus nigra
flavanone 3-dioxygenase
-
 Prunus cerasus
flavanone 3-dioxygenase
-
 Ribes uva-crispa
flavanone 3-hydroxylase
-
 Prunus avium
flavanone 3-hydroxylase
-
 Rubus plicatus
flavanone 3-hydroxylase
-
 Prunus domestica
flavanone 3-hydroxylase
-
 Rubus idaeus
flavanone 3-hydroxylase
-
 Sambucus nigra
flavanone 3-hydroxylase
-
 Prunus cerasus
flavanone 3-hydroxylase
-
 Ribes uva-crispa

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
 Prunus avium
2-oxoglutarate
-
 Rubus plicatus
2-oxoglutarate
-
 Prunus domestica
2-oxoglutarate
-
 Rubus idaeus
2-oxoglutarate
-
 Sambucus nigra
2-oxoglutarate
-
 Prunus cerasus
2-oxoglutarate
-
 Ribes uva-crispa
ascorbate
-
 Prunus avium
ascorbate
-
 Rubus plicatus
ascorbate
-
 Prunus domestica
ascorbate
-
 Rubus idaeus
ascorbate
-
 Sambucus nigra
ascorbate
-
 Prunus cerasus
ascorbate
-
 Ribes uva-crispa
heme
-
 Prunus avium
heme
-
 Rubus plicatus
heme
-
 Prunus domestica
heme
-
 Rubus idaeus
heme
-
 Sambucus nigra
heme
-
 Prunus cerasus
heme
-
 Ribes uva-crispa