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Literature summary for 1.14.11.74 extracted from

  • Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
    A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis (2013), MicrobiologyOpen, 2, 471-481 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pantoea ananatis
expression in Escherichia coli Pantoea ananatis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Pantoea ananatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2 Pantoea ananatis
-
 (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir
L-isoleucine + 2-oxoglutarate + O2 Pantoea ananatis AJ13355
-
 (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir

Organism

Organism UniProt Comment Textmining
Pantoea ananatis
-
-
-
Pantoea ananatis AJ13355
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 200 HR 10/30A column Pantoea ananatis

Storage Stability

Storage Stability Organism
-70°C Pantoea ananatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir
L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis AJ13355 (3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
 ir
L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis 31-hydroxy-L-isoleucine + succinate + CO2
-
 ?
L-isoleucine + 2-oxoglutarate + O2
-
 Pantoea ananatis AJ13355 31-hydroxy-L-isoleucine + succinate + CO2
-
 ?
L-methionine + 2-oxoglutarate + O2 low activity Pantoea ananatis L-methionine sulfoxide + succinate + CO2
-
 ?
L-methionine + 2-oxoglutarate + O2 low activity Pantoea ananatis AJ13355 L-methionine sulfoxide + succinate + CO2
-
 ?
L-valine + 2-oxoglutarate + O2 low activity Pantoea ananatis 4-hydroxy-L-valine + succinate + CO2
-
 ?
L-valine + 2-oxoglutarate + O2 low activity Pantoea ananatis AJ13355 4-hydroxy-L-valine + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
HilA
-
 Pantoea ananatis

General Information

General Information Comment Organism
physiological function enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine Pantoea ananatis