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Literature summary for 1.14.11.70 extracted from

  • Mazmouz, R.; Essadik, I.; Hamdane, D.; Mejean, A.; Ploux, O.
    Characterization of CyrI, the hydroxylase involved in the last step of cylindrospermopsin biosynthesis Binding studies, site-directed mutagenesis and stereoselectivity (2018), Arch. Biochem. Biophys., 647, 1-9 .
    View publication on PubMed
Search for more literature for 1.14.11.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Oscillatoria sp. PCC 7926

Protein Variants

Protein Variants Comment Organism
D159A inactive Oscillatoria sp. PCC 7926
H157A inactive Oscillatoria sp. PCC 7926
H247A inactive Oscillatoria sp. PCC 7926
R257A inactive Oscillatoria sp. PCC 7926

Inhibitors

Inhibitors Comment Organism Structure
7-deoxycylindrospermopsin the enzyme is strongly inhibited by 7-deoxycylindrospermopsin at concentration higher than 0.001 mM Oscillatoria sp. PCC 7926

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
 2-oxoglutarate pH 7.4 and 28°C Oscillatoria sp. PCC 7926
0.019
-
 7-deoxycylindrospermopsin pH 7.4 and 28°C Oscillatoria sp. PCC 7926

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on. Km value is 0.0021 mM. 0.05 mM used in assay conditions Oscillatoria sp. PCC 7926

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 cylindrospermopsin + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Oscillatoria sp. PCC 7926
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap nickel column chromatography and Superdex S200 gel filtration Oscillatoria sp. PCC 7926

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 cylindrospermopsin + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
cyrI
-
 Oscillatoria sp. PCC 7926

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 2-oxoglutarate pH 7.4 and 28°C Oscillatoria sp. PCC 7926

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
38.3
-
 7-deoxycylindrospermopsin pH 7.4 and 28°C Oscillatoria sp. PCC 7926