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Literature summary for 1.14.11.7 extracted from

  • Tiainen, P.; Pasanen, A.; Sormunen, R.; Myllyharju, J.
    Characterization of recombinant human prolyl 3-hydroxylase isoenzyme 2, an enzyme modifying the basement membrane collagen IV (2008), J. Biol. Chem., 283, 19432-19439.
    View publication on PubMed
Search for more literature for 1.14.11.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of both isoforms P3H1, P3H2 in Sf9 cell Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
poly(L-Pro) MW 7000-8000 Da; MW 7000-8000 Da Homo sapiens
Pyridine-2,4-dicarboxylate
-
 Homo sapiens
Pyridine-2,5-dicarboxylate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 (Gly-L-Pro-L-4-hydroxyproline)5 isoform P3H2, pH 7.8, 37°C Homo sapiens
0.07
-
 L-Pro-L-Thr-Gly-L-Pro-L-Arg-Gly-L-Phe-L-Pro-Gly-L-Pro-L-4-hydroxyproline-Gly-L-Pro-L-Asp-Gly-L-Leu-L-4-hydroxyproline-Gly-L-Ser-L-Met-Gly isoform P3H2, pH 7.8, 37°C Homo sapiens
0.08
-
 2-oxoglutarate isoform P3H2, pH 7.8, 37°C Homo sapiens
0.26
-
 L-Ser-L-Lys-Gly-L-Glu-L-Gln-Gly-L-Phe-L-Met-Gly-L-Pro-L-4-hydroxyproline-Gly-L-Pro-L-Gln-Gly-L-Gln-L-4-hydroyproline-Gly-L-Leu-L-4-hydroxyproline-Gly isoform P3H2, pH 7.8, 37°C Homo sapiens
0.26
-
 L-Leu-L-Asn-Gly-L-Leu-L-4Hyp-Gly-L-Pro-L-Ile-Gly-L-Pro-L-4Hyp-Gly-L-Pro-L-Arg-Gly-L-Arg-L-Thr-Gly-L-Asp-L-Ala-Gly isoform P3H2, pH 7.8, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Km value 0.0005 mM Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens isoform P3H2 is responsible for the hydroxylation of collagen IV ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q32P28 isoforms P3H1
-
Homo sapiens Q8IVL5 isoform P3H2
-

Purification (Commentary)

Purification (Comment) Organism
most of the recombinant isoform P3H2 is insoluble. Coexpression of protein with the cartilage-associated protein CRTAP does not enhance solubility Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
additional information isoform P3H2 is expressed in all tissues studied except for brain and skeletal muscle, with strong expression in tissues rich in basement membranes Homo sapiens
-
pancreas
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Gly-L-Pro-L-4-hydroxyproline)5 + 2-oxoglutarate + O2
-
 Homo sapiens (Gly-trans-3-hydroxy-L-Pro-trans-4-hydrox-L-Pro)5 + succinate + CO2
-
 ?
(L-Pro-trans-4-hydroxy-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Homo sapiens (trans-3-hydroxy-L-Pro-trans-4-hydroxy-L-Pro-Gly)5 + succinate + CO2
-
 ?
L-Leu-L-Asn-Gly-L-Leu-L-4Hyp-Gly-L-Pro-L-Ile-Gly-L-Pro-L-4Hyp-Gly-L-Pro-L-Arg-Gly-L-Arg-L-Thr-Gly-L-Asp-L-Ala-Gly + 2-oxoglutarate + O2 peptide corresponding to the only prolyl 3-hydroxylation site in the alpha1 chain of collagen I Homo sapiens L-Leu-L-Asn-Gly-L-Leu-L-4Hyp-Gly-trans-3-hydroxy-L-Pro-L-Ile-Gly-L-Pro-L-4Hyp-Gly-trans-3-hydroxy-L-Pro-L-Arg-Gly-L-Arg-L-Thr-Gly-L-Asp-L-Ala-Gly + succinate + CO2
-
 ?
L-Pro-L-Thr-Gly-L-Pro-L-Arg-Gly-L-Phe-L-Pro-Gly-L-Pro-L-4-hydroxyproline-Gly-L-Pro-L-Asp-Gly-L-Leu-L-4-hydroxyproline-Gly-L-Ser-L-Met-Gly + 2-oxoglutarate + O2 peptide corresponding to a known prolyl 3-hydroxylation site in the alpha1 chain of collagen IV Homo sapiens ? + succinate + CO2
-
 ?
L-Ser-L-Lys-Gly-L-Glu-L-Gln-Gly-L-Phe-L-Met-Gly-L-Pro-L-4-hydroxyproline-Gly-L-Pro-L-Gln-Gly-L-Gln-L-4-hydroyproline-Gly-L-Leu-L-4-hydroxyproline-Gly + 2-oxoglutarate + O2 peptide corresponding to a known prolyl 3-hydroxylation site in the alpha1 chain of collagen IV Homo sapiens ? + succinate + CO2
-
 ?
additional information isoform P3H2 is responsible for the hydroxylation of collagen IV Homo sapiens ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ascorbate Km value 0.11 mM Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.009
-
 Pyridine-2,4-dicarboxylate isoform P3H2, pH 7.8, 37°C Homo sapiens
1
-
 Pyridine-2,4-dicarboxylate isoform P3H2, pH 7.8, 37°C Homo sapiens
8
-
 poly(L-Pro) isoform P3H2, pH 7.8, 37°C Homo sapiens