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Literature summary for 1.14.11.69 extracted from

  • Hancock, R.L.; Masson, N.; Dunne, K.; Flashman, E.; Kawamura, A.
    The activity of JmjC histone lysine demethylase KDM4A is highly sensitive to oxygen concentrations (2017), ACS Chem. Biol., 12, 1011-1019 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.69

Application

Application Comment Organism
analysis KDM4A possesses the potential to act as an oxygen sensor in the context of chromatin modifications, with possible implications for epigenetic regulation in hypoxic disease states Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM4A, recombinant overexpression in U2O2 cells, recombinant expression of N-terminally FLAG-tagged KDM4A or KDM4A mutant H188A in HeLa cells, recombinant expression of N-terminally His6-tagged truncated KDM4A1-359 in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
H2O2 loss of KDM4A activity in hypoxia resulting in changes to global histone lysine methylation Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75164
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged truncated KDM4A1-359 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme is active on H3K9me3/me2 and H3K36me3/me2 substrates. The cellular activity of recombinant KDM4A against its primary substrate, H3K9me3, displays a graded response to depleting oxygen concentrations in line with the data obtained using isolated protein Homo sapiens ?
-
 ?
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
JmjC histone lysine demethylase
-
 Homo sapiens
JMJD2A
-
 Homo sapiens
KDM4A
-
 Homo sapiens
More see also EC 1.14.11.66 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information alteration of multiple H3 methylation marks by KDM4A at different oxygen concentrations, overview Homo sapiens

General Information

General Information Comment Organism
metabolism KDM4A possesses the potential to act as an oxygen sensor in the context of epigenetic regulation Homo sapiens
additional information cellular demethylase activity of KDM4A demonstrates a graded response to oxygen concentration in U2OS cells. Analysis of the H3K27me3 (cf. EC 1.14.11.68) mark shows loss of this mark upon overexpression of KDM4A in normoxia, with a graded response to oxygen similar to that seen for H3K9me3, although less-pronounced. H3K27me3 is not a canonical substrate for KDM4A, hence, loss of this mark cannot be directly attributed to catalytic KDM4A activity. Effect of oxygen availability on the activity of the KDM4 subfamily member KDM4A, overview. A high level of O2 sensitivity both with isolated protein and in cells is observed Homo sapiens
physiological function the JmjC histone lysine demethylases (KDMs) are epigenetic regulators involved in the removal of methyl groups from post-translationally modified lysyl residues within histone tails, modulating gene transcription Homo sapiens