Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene KDM4A, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
gene KDM4B, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
gene KDM4C, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for catalysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75164 | - |
- |
Homo sapiens | O94953 | - |
- |
Homo sapiens | Q9H3R0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and dialysis | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00029 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate H3K9me3 | Homo sapiens |
0.0004 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K297me3 | Homo sapiens |
0.0005 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate H3K9me3 | Homo sapiens |
0.0006 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K448me3 | Homo sapiens |
0.00064 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K297me3 | Homo sapiens |
0.00095 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CDYL1-K135me3 | Homo sapiens |
0.001 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CDYL1-K135me3 | Homo sapiens |
0.001 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate H3K9me3 | Homo sapiens |
0.0011 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K170me3 | Homo sapiens |
0.00143 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K1054me3 | Homo sapiens |
0.0015 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K297me3 | Homo sapiens |
0.00163 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K1054me3 | Homo sapiens |
0.00188 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate WIZ-K305me3 | Homo sapiens |
0.0019 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate CSB-K1054me3 | Homo sapiens |
0.0019 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K170me3 | Homo sapiens |
0.002 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K170me3 | Homo sapiens |
0.00212 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate G9a-K185me3 | Homo sapiens |
0.00232 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CSB-K448me3 | Homo sapiens |
0.0024 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate G9a-K185me3 | Homo sapiens |
0.0025 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate CSB-K448me3 | Homo sapiens |
0.0026 | - |
recombinant isozyme JMJD2A, pH 7.5, 22°C, substrate G9a-K185me3 | Homo sapiens |
0.0031 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate WIZ-K305me3 | Homo sapiens |
0.0034 | - |
recombinant isozyme JMJD2C, pH 7.5, 22°C, substrate CDYL1-K135me3 | Homo sapiens |
0.0035 | - |
recombinant isozyme JMJD2B, pH 7.5, 22°C, substrate WIZ-K305me3 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDYL1-K135me3 + 2-oxoglutarate + O2 | from chromodomain Y-like protein | Homo sapiens | CDYL1-K135me2 + succinate + formaldehyde + CO2 | - |
? | |
CSB-K1054me3 + 2-oxoglutarate + O2 | CBS is the Cockayne syndrome group B protein | Homo sapiens | CSB-K1054me2 + succinate + formaldehyde + CO2 | - |
? | |
CSB-K170me3 + 2-oxoglutarate + O2 | CBS is the Cockayne syndrome group B protein | Homo sapiens | CSB-K170me2 + succinate + formaldehyde + CO2 | - |
? | |
CSB-K297me3 + 2-oxoglutarate + O2 | CBS is the Cockayne syndrome group B protein | Homo sapiens | CSB-K297me2 + succinate + formaldehyde + CO2 | - |
? | |
CSB-K448me3 + 2-oxoglutarate + O2 | CBS is the Cockayne syndrome group B protein | Homo sapiens | CSB-K448me2 + succinate + formaldehyde + CO2 | - |
? | |
G9a-K185me3 + 2-oxoglutarate + O2 | - |
Homo sapiens | G9a-K185me2 + succinate + formaldehyde + CO2 | - |
? | |
additional information | the bifunctional enzyme is active on H3K9me3/me2 and H3K36me3/me2 (EC 1.14.11.69) substrates, and also on non-histone substrates, substrate specificities of isozymes JMJD2A-C, overview | Homo sapiens | ? | - |
? | |
WIZ-K305me3 + 2-oxoglutarate + O2 | from widely interspaced zinc finger motifs protein | Homo sapiens | WIZ-K305me2 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
histone demethylase | - |
Homo sapiens |
JMJD2A | - |
Homo sapiens |
JMJD2B | - |
Homo sapiens |
JMJD2C | - |
Homo sapiens |
KDM4A | - |
Homo sapiens |
KDM4B | - |
Homo sapiens |
Kdm4c | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |