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Literature summary for 1.14.11.66 extracted from

  • Jang, M.K.; Kim, J.H.; Jung, M.H.
    Histone H3K9 demethylase JMJD2B activates adipogenesis by regulating H3K9 methylation on PPARgamma and C/EBPalpha during adipogenesis (2017), PLoS ONE, 12, e0168185 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM4B, quantitative real-time PCR enzyme expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme knockout by expression of siRNA targeting JMJD2B in 3T3-L1 preadipocytes Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
-
?
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O94953
-
-

Source Tissue

Source Tissue Comment Organism Textmining
3T3-L1 cell
-
Homo sapiens
-
preadipocyte
-
Homo sapiens
-
white adipose tissue
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2
-
Homo sapiens histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
-
?
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
-
Homo sapiens histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
?

Synonyms

Synonyms Comment Organism
histone H3K9 demethylase
-
Homo sapiens
JMJD2B
-
Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme JMJD2B belongs to the jumonji C (JmjC) domain-containing, iron-dependent dioxygenases (JMJC demethylases) Homo sapiens
malfunction JMJD2B knockdown using siRNA in 3T3-L1 preadipocytes represses the expression of PPARgamma and C/EBPalpha, resulting in inhibition of adipogenesis. This is accompanied by increased enrichment of H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha. In contrast, overexpression of JMJD2B increases the expression of PPARgamma and C/EBPalpha, which is accompanied by decreased enrichment of H3K9me3/me2 on the promoter and activated adipogenesis Homo sapiens
metabolism the methylation of lysine residues in histones is a main epigenetic modification in the regulation of eukaryotic gene expression. While methylation of histone H3 at the lysine 4 (H3K4) and 36 (H3K36) residues primarily associates with active transcription, methylation of histone H3 at the lysine 9 (H3K9) and 27 (H3K27) residues and histone H4 at lysine 20 (H4K20) associates with gene repression. Histone methylation by histone methyltransferase is antagonized by histone demethylases, which are divided into two classes: amine oxidases (LSD demethylases), and jumonji C (JmjC) domain-containing, iron-dependent dioxygenases (JMJC demethylases). Histone H3K9 demethylase JMJD2B decreases H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha, which in turn stimulated the expression of PPARgamma and C/EBPalpha. JMJD2B-mediated reduction of H3K9me3 increases H3K4me3 level on PPARgamma and C/EBPalpha Homo sapiens
physiological function histone H3K9 demethylase JMJD2B activates adipogenesis by regulating H3K9 methylation on PPARgamma and C/EBPalpha during adipogenesis in 3T3-L1 preadipocytes, overview. JMJD2B decreases H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha, which in turn stimulated the expression of PPARgamma and C/EBPalpha Homo sapiens