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Literature summary for 1.14.11.66 extracted from

  • Ponnaluri, V.; Vavilala, D.; Putty, S.; Gutheil, W.; Mukherji, M.
    Identification of non-histone substrates for JMJD2A-C histone demethylases (2009), Biochem. Biophys. Res. Commun., 390, 280-284.
    View publication on PubMed
Search for more literature for 1.14.11.66

Cloned(Commentary)

Cloned (Comment) Organism
JMJD2A-C genes, DNA and amino acid sequence determination and analysis, expression of the GST-tagged isozymes JMJD2A, JMJD2B, and JMJD2C in Escherichia coli strain BL21(DE3) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2 Homo sapiens
-
 [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozymes JMJD2A, JMJD2B, and JMJD2C
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged isozymes JMJD2A, JMJD2B, and JMJD2C from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific isozyme activities with non-histone substrates, overview Homo sapiens
0.00029
-
 recombinant purified isozyme JMJD2B, substrate histone H3K9 Homo sapiens
0.0005
-
 recombinant purified isozyme JMJD2C, substrate histone H3K9 Homo sapiens
0.001
-
 recombinant purified isozyme JMJD2A, substrate histone H3K9 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of recombinant isozymes, overview Homo sapiens ?
-
 ?
[chromodomain Y-like protein]-N6,N6,N6-trimethyl-L-lysine135 + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
[Cockayne syndrome group B protein]-N6,N6,N6-trimethyl-L-lysine1054 + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
[Cockayne syndrome group B protein]-N6,N6,N6-trimethyl-L-lysine170 + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
[Cockayne syndrome group B protein]-N6,N6,N6-trimethyl-L-lysine297 + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
[Cockayne syndrome group B protein]-N6,N6,N6-trimethyl-L-lysine448 + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?
[G9a protein]-N6,N6,N6-trimethyl-L-lysine185 + 2-oxoglutarate + O2 a customer synthesized protein Homo sapiens ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2
-
 Homo sapiens [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2 isozyme JMJD2A substrate binding structure, overview Homo sapiens [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2
-
 ?
[widely interspaced zinc finger motifs protein]-N6,N6,N6-trimethyl-L-lysine305 + 2-oxoglutarate + O2
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
histone demethylase
-
 Homo sapiens
JMJD2A
-
 Homo sapiens
JMJD2B
-
 Homo sapiens
JMJD2C
-
 Homo sapiens
More the enzyme belongs to the Jumonji domain-2 family histone demethylases Homo sapiens