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Literature summary for 1.14.11.65 extracted from
- Control of histone H3 lysine 9 (H3K9) methylation state via cooperative two-step demethylation by jumonji domain containing 1A (JMJD1A) homodimer (2013), J. Biol. Chem., 288, 36948-36956 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in HEK293T cell | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H1120Y | inactive | Homo sapiens |
| H1120Y | loss of enzymatic activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.095 | - |
[histone H3]-N6-methyl-L-lysine9 | pH 8.0, 37°C | Homo sapiens |  |
| 0.0952 | - |
[histone H3]-N6-methyl-L-lysine9 | at pH 8.0 and 37°C | Homo sapiens | |
| 0.106 | - |
[histone H3]-N6,N6-dimethyl-L-lysine9 | pH 8.0, 37°C | Homo sapiens | |
| 0.1061 | - |
[histone H3]-N6,N6-dimethyl-L-lysine9 | at pH 8.0 and 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 300000 | - |
gel filtration | Homo sapiens |
| 300000 | - |
PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | no activity with [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | ? | - |
- |
|
| [histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2 | Homo sapiens | overall reaction | [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| [histone H3]-N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
| [histone H3]-N6-methyl-L-lysine9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q9Y4C1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Streptactin-Sepharose column chromatography and FLAG M2-agarose column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293T cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with [histone H3]-N6,N6,N6-trimethyl-L-lysine9 | Homo sapiens | ? | - |
- |
|
| [histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2 | - |
Homo sapiens | [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| [histone H3]-N6,N6-dimethyl-L-lysine9 + 2 2-oxoglutarate + 2 O2 | overall reaction | Homo sapiens | [histone H3]-L-lysine9 + 2 succinate + 2 formaldehyde + 2 CO2 | - |
? | |
| [histone H3]-N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
| [histone H3]-N6-methyl-L-lysine9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-L-lysine9 + succinate + formaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 150000, calculated from sequence | Homo sapiens |
| homodimer | 2 * 150000, SDS-PAGE | Homo sapiens |
| More | two active sites of a JMJD1A dimer are required for effective demethylation | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| JMJD1A | - |
Homo sapiens |
| JMJD1A | Jumonji domain containing 1A, gene name | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.275 | - |
[histone H3]-N6,N6-dimethyl-L-lysine9 | pH 8.0, 37°C | Homo sapiens | |
| 0.275 | - |
[histone H3]-N6,N6-dimethyl-L-lysine9 | at pH 8.0 and 37°C | Homo sapiens | |
| 0.282 | - |
[histone H3]-N6-methyl-L-lysine9 | pH 8.0, 37°C | Homo sapiens | |
| 0.282 | - |
[histone H3]-N6-methyl-L-lysine9 | at pH 8.0 and 37°C | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | JMJD1A forms a homodimer through its catalytic domains, bringing the two active sites close together. Increasing the concentration of JMJD1A facilitates efficient production of unmethylated product from dimethyl-H3K9 and decreases the release of the monomethylated intermediate. Substituting one of the two active sites with an inactive mutant results in a significant reduction of the demethylation rate without changing the affinity to the intermediate. A substrate channeling model is described for the efficient conversion of dimethylated H3K9 into the unmethylated state | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.58 | - |
[histone H3]-N6,N6-dimethyl-L-lysine9 | pH 8.0, 37°C | Homo sapiens | |
| 2.58 | - |
[histone H3]-N6,N6-dimethyl-L-lysine9 | at pH 8.0 and 37°C | Homo sapiens | |
| 2.97 | - |
[histone H3]-N6-methyl-L-lysine9 | pH 8.0, 37°C | Homo sapiens | |
| 2.97 | - |
[histone H3]-N6-methyl-L-lysine9 | at pH 8.0 and 37°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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