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Literature summary for 1.14.11.65 extracted from

  • Janzer, A.; Lim, S.; Fronhoffs, F.; Niazy, N.; Buettner, R.; Kirfel, J.
    Lysine-specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1) synergistically repress proinflammatory cytokines and classical complement pathway components (2012), Biochem. Biophys. Res. Commun., 421, 665-670 .
    View publication on PubMed
Search for more literature for 1.14.11.65

Protein Variants

Protein Variants Comment Organism
additional information MDA-MB231 cells are transiently transfected with three different siRNA directed against LSD1 causing significant knockdown of LSD1 in all samples on both mRNA and protein level. LSD1 knockdown leads to significant induction of the steady state transcript levels of interleukin 1alpha, 1beta, 6 and 8 genes. Interleukin 1beta protein levels are significantly enhanced in the supernatant of the cells after LSD1 knockdown Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
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 [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60341
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Hep-G2 cell
-
 Homo sapiens
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additional information LSD1 microarray transcriptome analysis, overview Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 and H3K4me2/me1 (EC 1.14.99.66) Homo sapiens ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
LSD1
-
 Homo sapiens
lysine-specific demethylase 1
-
 Homo sapiens
More see also for EC 1.14.99.66 Homo sapiens

General Information

General Information Comment Organism
malfunction knockdown of LSD1 diminishes LSD1 occupancy on the IL1beta and the IL6 promoter, while promoter occupancy on those promoters cannot be detected with an IgG control antibody. Inhibition of LSD1 by an siRNA approach is accompanied by an increase of the active histone mark H3K4me2 and a decrease in the repressive H3K9me2 mark, resulting in activation of IL1beta and IL6 genes after LSD1 silencing Homo sapiens
metabolism LSD1 microarray transcriptome analysis, overview Homo sapiens
physiological function the catalytic activity of lysine-specific demethylase 1 (LSD1) is required for regulation of inflammatory cytokines. LSD1 functions as a transcriptional coregulator through demethylating histone H3 on lysine 4 and lysine 9. Repressive role of LSD1 in proinflammatory cytokine expression such as interleukins IL1alpha, IL1beta, IL6, and IL8 and classical complement components, LSD1 occupies and regulates the promoter of these genes. LSD1 regulates several genes of the complement system in Hep-G2 cells. LSD1 binds directly to the promoter of the IL1beta and IL6 genes Homo sapiens