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Literature summary for 1.14.11.65 extracted from

  • El Mansouri, F.; Nebbaki, S.; Kapoor, M.; Afif, H.; Martel-Pelletier, J.; Pelletier, J.; Benderdour, M.; Fahmi, H.
    Lysine-specific demethylase 1-mediated demethylation of histone H3 lysine 9 contributes to interleukin 1beta-induced microsomal prostaglandin E synthase 1 expression in human osteoarthritic chondrocytes (2014), Arthritis Res. Ther., 16, R113 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.65

Activating Compound

Activating Compound Comment Organism Structure
additional information interleukin-1beta enhances recruitment of LSD1 to mPGES-1 promoter Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM1A, real-time PCR enzyme expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the enzyme is knocked out by expression of specific siRNA for LSD1 in chondrocytes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Pargyline
-
 Homo sapiens
tranylcypromine
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatin
-
 Homo sapiens 785
-
mitochondrion enzyme LSD1 is present at the proximal region of the microsomal PGES-1 promoter Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 L-lysine9 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60341
-
-

Source Tissue

Source Tissue Comment Organism Textmining
chondrocyte osteoarthritic chondrocytes Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 L-lysine9 + succinate + formaldehyde + CO2
-
 ?
additional information histone H3 N6,N6,N6-trimethyl-L-lysine9 is no substrate Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
BHC110
-
 Homo sapiens
KDM1A
-
 Homo sapiens
LSD1
-
 Homo sapiens
lysine-specific demethylase 1
-
 Homo sapiens
NPAO
-
 Homo sapiens
p110b
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD required, LSD1 utilizes FAD as an essential cofactor in catalyzing demethylation of mono- and di-methylated H3K9. Interleukin-1-induced H3K9 demethylation and LSD1 recruitment in human chondrocytes are not associated with significant changes in FAD levels Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the superfamily of flavin adenine dinucleotide (FAD)–dependent amine oxidases Homo sapiens
malfunction both pharmacological inhibition of LSD1 and small interfering RNA (siRNA) knockdown prevents interleukin 1beta-induced H3K9 demethylation at the mPGES-1 promoter as well as concomitant mPGES-1 protein expression. The level of LSD1 expression is elevated in osteoarthritis cartilage Homo sapiens
physiological function lysine-specific demethylase 1-mediated demethylation of histone H3 lysine 9 contributes to interleukin 1beta-induced microsomal prostaglandin E synthase 1 (mPGES-1) expression in human osteoarthritic chondrocytes. mPGES-1 catalyzes the terminal step in the biosynthesis of PGE2, a critical mediator in the pathophysiology of osteoarthritis. Histone methylation plays an important role in epigenetic gene regulation. LSD1 modulates gene expression through demethylation of either H3K4 or H3K9. H3K9 methylation usually suppresses transcription, whereas H3K4 methylation generally activates transcription. The induction of mPGES-1 expression by interleukin-1beta is associated with decreased levels of mono- and dimethylated H3K9, but not trimethylated H3K9, at the mPGES-1 promoter correlating with the recruitment of the histone demethylase LSD1 Homo sapiens