Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + 2-oxoglutarate + O2 | Leishmania major | - |
5-hydroxymethyluracil + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q4QFY1 | - |
- |
Leishmania major | Q4QHM7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + 2-oxoglutarate + O2 | - |
Leishmania major | 5-hydroxymethyluracil + succinate + CO2 | - |
? | |
thymine + 2-oxoglutarate + O2 | the telomeric repeat sequence GGGTTA is able to stimulate J synthesis, while mutated telomeric sequences (GGGTTT, GGGATT, or GGGAAA) are unable to support J synthesis | Leishmania major | 5-hydroxymethyluracil + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
JBP1 | - |
Leishmania major |
JBP2 | - |
Leishmania major |
thymidine hydroxylase | - |
Leishmania major |
General Information | Comment | Organism |
---|---|---|
physiological function | telomeric DNA of Trypanosomatids possesses a modified thymine base, called base J or beta-D-glucopyranosyloxymethyluracil, that is synthesized in a two-step process. The base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU), a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). Both JBP1 and JBP2 stimulate de novo thymidine hydroxylation in vivo. DNA Jaylation is largely regulated by cis-acting sequences and is thus genetically encoded. The key regulatory step of J synthesis seems to be the first step catalyzed by JBP1 and JBP2. The specificity of base J localization is probably due to the JBP enzymes generating 5-hydroxymethyluracil at only specific sites throughout the genome | Leishmania major |