Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.6 extracted from

  • Warn-Cramer, B.J.; Macrander, L.A.; Abbott, M.T.
    Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis (1983), J. Biol. Chem., 258, 10551-10557.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate at 3 mM 5fold stimulation of the thymine and the 5-formyluracil-dependent reactions, only 15% stimulation of the 5-hydroxymethyluracil dependent reaction Rhodotorula glutinis
ATP at 5 mM 4fold stimulation of the thymine and 5-formyluracil-dependent reactions, only 20% stimulation of the 5-hydroxymethyluracil dependent reaction Rhodotorula glutinis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Rhodotorula glutinis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40300
-
disc gel electrophoresis under denaturing conditions Rhodotorula glutinis
42700
-
1 * 42700, gel filtration Rhodotorula glutinis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-formyluracil + 2-oxoglutarate + O2 Rhodotorula glutinis
-
5-carboxyuracil + succinate + CO2
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2 Rhodotorula glutinis
-
5-formyluracil + succinate + CO2
-
?
thymine + 2-oxoglutarate + O2 Rhodotorula glutinis
-
5-hydroxymethyluracil + succinate + CO2 5-hydroxymethyluracil reacts subsequently to 5-formyluracil ?

Organism

Organism UniProt Comment Textmining
Rhodotorula glutinis
-
grown with thymine as nitrogen source
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodotorula glutinis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.1
-
-
Rhodotorula glutinis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-formyluracil + 2-oxoglutarate + O2
-
Rhodotorula glutinis 5-carboxyuracil + succinate + CO2
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
-
Rhodotorula glutinis 5-formyluracil + succinate + CO2
-
?
thymine + 2-oxoglutarate + O2
-
Rhodotorula glutinis 5-hydroxymethyluracil + succinate + CO2 5-hydroxymethyluracil reacts subsequently to 5-formyluracil ?

Subunits

Subunits Comment Organism
monomer 1 * 42700, gel filtration Rhodotorula glutinis