Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | at 3 mM 5fold stimulation of the thymine and the 5-formyluracil-dependent reactions, only 15% stimulation of the 5-hydroxymethyluracil dependent reaction | Rhodotorula glutinis | |
ATP | at 5 mM 4fold stimulation of the thymine and 5-formyluracil-dependent reactions, only 20% stimulation of the 5-hydroxymethyluracil dependent reaction | Rhodotorula glutinis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Rhodotorula glutinis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40300 | - |
disc gel electrophoresis under denaturing conditions | Rhodotorula glutinis |
42700 | - |
1 * 42700, gel filtration | Rhodotorula glutinis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyluracil + 2-oxoglutarate + O2 | Rhodotorula glutinis | - |
5-carboxyuracil + succinate + CO2 | - |
? | |
5-hydroxymethyluracil + 2-oxoglutarate + O2 | Rhodotorula glutinis | - |
5-formyluracil + succinate + CO2 | - |
? | |
thymine + 2-oxoglutarate + O2 | Rhodotorula glutinis | - |
5-hydroxymethyluracil + succinate + CO2 | 5-hydroxymethyluracil reacts subsequently to 5-formyluracil | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula glutinis | - |
grown with thymine as nitrogen source | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula glutinis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.1 | - |
- |
Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyluracil + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | 5-carboxyuracil + succinate + CO2 | - |
? | |
5-hydroxymethyluracil + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | 5-formyluracil + succinate + CO2 | - |
? | |
thymine + 2-oxoglutarate + O2 | - |
Rhodotorula glutinis | 5-hydroxymethyluracil + succinate + CO2 | 5-hydroxymethyluracil reacts subsequently to 5-formyluracil | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42700, gel filtration | Rhodotorula glutinis |