Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | presence of catalase causes 20-30% increase in the turnover of L-proline | Streptomyces griseoviridis | |
pyridine-2,6-dicarboxylate | - |
Streptomyces griseoviridis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | inhibitory above 0.5 mM | Streptomyces griseoviridis | |
3,4-dihydroxybenzoate | - |
Streptomyces griseoviridis | |
Co2+ | 0.5 mM, complete inhibition | Streptomyces griseoviridis | |
diethyl dicarbonate | complete inhibition. Inactivation can be partially prevented by the inclusion of L-proline and 2-oxoglutarate in the preincubation mixture | Streptomyces griseoviridis | |
L-ascorbate | ascorbate causes a time-dependent inhibition of L-proline hydroxylation. The addition of ascorbate does not stimulate L-proline-coupled turnover of 2-oxoglutarate, but does stimulate L-proline-uncoupled turnover | Streptomyces griseoviridis | |
Pyridine-2,4-dicarboxylate | competitive with respect to 2-oxoglutarate | Streptomyces griseoviridis | |
Pyridine-2,5-dicarboxylate | - |
Streptomyces griseoviridis | |
Zn2+ | 0.5 mM, complete inhibition | Streptomyces griseoviridis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
2-oxoglutarate | pH 7.5, 35°C | Streptomyces griseoviridis | |
0.445 | - |
L-proline | pH 7.5, 35°C | Streptomyces griseoviridis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | about 10-15% of the activity with iron | Streptomyces griseoviridis | |
Iron | strict requirements for ferrous ion and 2-oxoglutarate, maximum activity at 0.03 mM | Streptomyces griseoviridis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, SDS-PAGE | Streptomyces griseoviridis |
38000 | - |
gel filtration | Streptomyces griseoviridis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseoviridis | - |
- |
- |
Streptomyces griseoviridis P8648 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
907 | - |
pH 7.5, 26°C | Streptomyces griseoviridis |
Storage Stability | Organism |
---|---|
-20°C, about 30% loss of activity by freezing and thawing | Streptomyces griseoviridis |
4°C, 4-5 h, stable | Streptomyces griseoviridis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces griseoviridis | trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces griseoviridis P8648 | trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
additional information | the enzyme displays strict requirements for ferrous ion and 2-oxoglutarate, no cosubstrate: 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate. L-Proline-uncoupled turnover of 2-oxoglutarate to succinate and CO2 is observed | Streptomyces griseoviridis | ? | - |
? | |
additional information | the enzyme displays strict requirements for ferrous ion and 2-oxoglutarate, no cosubstrate: 2-oxopentanoate, 2-oxoadipate, pyruvate or 2-oxomalonate. L-Proline-uncoupled turnover of 2-oxoglutarate to succinate and CO2 is observed | Streptomyces griseoviridis P8648 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Streptomyces griseoviridis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.005 | - |
pH 7.5, 35°C | Streptomyces griseoviridis | Pyridine-2,4-dicarboxylate | |
0.032 | - |
pH 7.5, 35°C | Streptomyces griseoviridis | 3,4-dihydroxybenzoate | |
0.049 | - |
pH 7.5, 35°C | Streptomyces griseoviridis | Pyridine-2,5-dicarboxylate |