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Literature summary for 1.14.11.55 extracted from

  • Widderich, N.; Pittelkow, M.; Höppner, A.; Mulnaes, D.; Buckel, W.; Gohlke, H.; Smits, S.; Bremer, E.
    Molecular dynamics simulations and structure-guided mutagenesis provide insight into the architecture of the catalytic core of the ectoine hydroxylase (2014), J. Mol. Biol., 426, 586-600 .
    View publication on PubMed
Search for more literature for 1.14.11.55

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Virgibacillus salexigens

Protein Variants

Protein Variants Comment Organism
A163C the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
A163C/S244C the mutant shows increased activity compared to the wild type enzyme Virgibacillus salexigens
D148A inactive Virgibacillus salexigens
D148E inactive Virgibacillus salexigens
F143A inactive Virgibacillus salexigens
F143W inactive Virgibacillus salexigens
F143Y inactive Virgibacillus salexigens
F242A inactive Virgibacillus salexigens
F242W inactive Virgibacillus salexigens
F242Y the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
F263A inactive Virgibacillus salexigens
F263W inactive Virgibacillus salexigens
F263Y the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
F95A inactive Virgibacillus salexigens
H146A inactive Virgibacillus salexigens
H248A inactive Virgibacillus salexigens
N133A inactive Virgibacillus salexigens
N261A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
P198A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
Q129A inactive Virgibacillus salexigens
R131A inactive Virgibacillus salexigens
R259A inactive Virgibacillus salexigens
R259H inactive Virgibacillus salexigens
R259K inactive Virgibacillus salexigens
R259Q inactive Virgibacillus salexigens
S165A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
S167A the mutant shows about wild type activity Virgibacillus salexigens
S205A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
S244C the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
S250A inactive Virgibacillus salexigens
V265A the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
V265L the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
V265T the mutant shows reduced activity compared to the wild type enzyme Virgibacillus salexigens
W152A inactive Virgibacillus salexigens
W152F inactive Virgibacillus salexigens
W152Y inactive Virgibacillus salexigens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5
-
 (4S)-ectoine mutant A163C/S244C, at pH 7.5 and 32°C Virgibacillus salexigens
5.5
-
 (4S)-ectoine mutant N261A, at pH 7.5 and 32°C Virgibacillus salexigens
5.9
-
 (4S)-ectoine wild type enzyme, at pH 7.5 and 32°C Virgibacillus salexigens
6
-
 (4S)-ectoine mutant A163C, at pH 7.5 and 32°C Virgibacillus salexigens
6.2
-
 (4S)-ectoine mutant S244C, at pH 7.5 and 32°C Virgibacillus salexigens
6.8
-
 (4S)-ectoine mutant S205A, at pH 7.5 and 32°C Virgibacillus salexigens
6.9
-
 (4S)-ectoine mutant S167A, at pH 7.5 and 32°C Virgibacillus salexigens
7.1
-
 (4S)-ectoine mutant P198A, at pH 7.5 and 32°C Virgibacillus salexigens
8
-
 (4S)-ectoine mutant F263Y, at pH 7.5 and 32°C Virgibacillus salexigens
9.7
-
 (4S)-ectoine mutant V265T, at pH 7.5 and 32°C Virgibacillus salexigens
16.2
-
 (4S)-ectoine mutant V265A, at pH 7.5 and 32°C Virgibacillus salexigens
17.3
-
 (4S)-ectoine mutant S165A, at pH 7.5 and 32°C Virgibacillus salexigens
19.6
-
 (4S)-ectoine mutant F242Y, at pH 7.5 and 32°C Virgibacillus salexigens
24.6
-
 (4S)-ectoine mutant V265L, at pH 7.5 and 32°C Virgibacillus salexigens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Virgibacillus salexigens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4S)-ectoine + 2-oxoglutarate + O2 Virgibacillus salexigens
-
 (4S,5S)-5-hydroxyectoine + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Virgibacillus salexigens Q2TDY4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4S)-ectoine + 2-oxoglutarate + O2
-
 Virgibacillus salexigens (4S,5S)-5-hydroxyectoine + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ectD
-
 Virgibacillus salexigens