Cloned (Comment) | Organism |
---|---|
a plasmid with the vector backbone pNIC28-Bsa4 encoding a hexahistidine-tagged ALKBH566292 construct is transformed into Escherichia coli BL21 (DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
ALKBH566292 is crystallized in sitting drops at 20°C by the vapour diffusion method in the presence of Mn2+ and (1-chloro-4-hydroxyisoquinoline-3-carbonyl)glycine. Crystallization drops contain 0.2 ml of a protein solution containing a final concentration of 10 mg/ml hexahistidine-tagged ALKBH566292, 0.5 mM MnCl2 and 2 mM IOX3 mixed with 0.1 ml of well solution containing 125 mM potassium nitrate and 15% (w/v) polyethylene glycol 3350. Crystals (size 100 x 50 x 50 mM) appeared after 3 months. Crystals are harvested using nylon loops and cryoprotected using well solution diluted with 25% (v/v) glycerol and flashcooled in liquid nitrogen. Crystal structure of human ALKBH5 (residues 66-292) to 2.0 A resolution. ALKBH566292 has a double-stranded beta-helix core fold. The active site metal is octahedrally coordinated by an HXD...H motif (comprising residues His204, Asp206 and His266) and three water molecules. ALKBH5 shares a nucleotide recognition lid and conserved active site residues with other ferrous iron-dependent nucleic acid oxygenase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1-chloro-4-hydroxyisoquinoline-3-carbonyl)glycine | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6P6C2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-methyladenine in mRNA + 2-oxoglutarate + O2 | - |
Homo sapiens | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALKBH5 | - |
Homo sapiens |
RNA N6-methyladenine demethylase | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | ALKBH5 is upregulated under hypoxia | up |
General Information | Comment | Organism |
---|---|---|
physiological function | plays a role in spermatogenesis | Homo sapiens |