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Literature summary for 1.14.11.45 extracted from

  • Zhang, C.; Ma, J.; Li, Z.; Liang, Y.; Xu, Q.; Xie, X.; Chen, N.
    A strategy for L-isoleucine dioxygenase screening and 4-hydroxyisoleucine production by resting cells (2018), Bioengineered, 9, 72-79 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis development of efficient method for the biotransformation of 4-hydroxyisoleucine by resting cells expressing L27I/E80D/G169H/S182D 151.9 mM of 4-hydroxyisoleucine/l (22.4 g/l) can be synthesized in 12 h while the substrates seldom exhibits additional consumption Bacillus thuringiensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus thuringiensis

Protein Variants

Protein Variants Comment Organism
L27I/E80D/G169H/S182D catalytic efficiency, thermal stability and catalytic rate are significantly improved compared with those of wild-type IDO Bacillus thuringiensis
additional information mutants with improved activity are obtained by a strategy dependent on the restoration of succinate-minus Escherichia coli cell growth by the coupling of L-Ile hydroxylation and the oxidation of 2-oxoglutarate to succinate Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
L-isoleucine mutant L27I/E80D/G169H/S182D, pH 7.0, 30┬░C Bacillus thuringiensis
0.2
-
L-isoleucine wild-type, pH 7.0, 30┬░C Bacillus thuringiensis

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis S5SPJ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2
-
Bacillus thuringiensis (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
IDO
-
Bacillus thuringiensis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
35
-
-
Bacillus thuringiensis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60
-
30 min, more than 60% residual activity Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.073
-
L-isoleucine wild-type, pH 7.0, 30┬░C Bacillus thuringiensis
0.084
-
L-isoleucine mutant L27I/E80D/G169H/S182D, pH 7.0, 30┬░C Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus thuringiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.36
-
L-isoleucine wild-type, pH 7.0, 30┬░C Bacillus thuringiensis
0.56
-
L-isoleucine mutant L27I/E80D/G169H/S182D, pH 7.0, 30┬░C Bacillus thuringiensis