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Literature summary for extracted from

  • Yu, X.; Shi, F.; Liu, H.; Tan, S.; Li, Y.
    Programming adaptive laboratory evolution of 4-hydroxyisoleucine production driven by a lysine biosensor in Corynebacterium glutamicum (2021), AMB Express, 11, 66 .
    View publication on PubMedView publication on EuropePMC


Application Comment Organism
synthesis improved synthesis of 4-HIL in an optimized strain of Corynebacterium glutamicum by application of programming adaptive laboratory evolution. The programming evolutionary system contains a Lys biosensor LysG-PlysE and an evolutionary actuator composed of a mutagenesis gene and a fluorescent protein gene. After successive rounds of evolution, mutant strains with significantly increased 4-HIL production and growth performance are obtained. The maximum 4-HIL titer is 152.19 mM, 28.4% higher than the starting strain Bacillus thuringiensis


Organism UniProt Comment Textmining
Bacillus thuringiensis E2GIN1