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Literature summary for 1.14.11.45 extracted from

  • Shi, F.; Niu, T.; Fang, H.
    4-Hydroxyisoleucine production of recombinant Corynebacterium glutamicum ssp. lactofermentum under optimal corn steep liquor limitation (2015), Appl. Microbiol. Biotechnol., 99, 3851-3863 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells and Corynebacterium glutamicum ssp. lactofermentum strain SN01 Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
2-oxoglutarate at pH 6.0 and 40┬░C Bacillus thuringiensis
0.61
-
L-isoleucine at pH 6.0 and 40┬░C Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis
-
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis YBT-1520
-
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis
-
-
-
Bacillus thuringiensis YBT-1520
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus thuringiensis

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:corn steep liquor-grown cell
-
Bacillus thuringiensis
-

Storage Stability

Storage Stability Organism
25┬░C, purified enzyme, 1 h and 4 h, 18% and more than 70% loss of activity, respectively Bacillus thuringiensis
4┬░C, purified enzyme, 2 and 8 days, 30% and more than 85% loss of activity, respectively Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2
-
Bacillus thuringiensis (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?
L-isoleucine + 2-oxoglutarate + O2
-
Bacillus thuringiensis YBT-1520 (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Bacillus thuringiensis
? x * 29550, calculated from amino acid sequence Bacillus thuringiensis

Synonyms

Synonyms Comment Organism
IDO
-
Bacillus thuringiensis
L-isoleucine dioxygenase
-
Bacillus thuringiensis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
40
-
-
Bacillus thuringiensis

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
50 70 preincubating the enzyme at 50┬░C for 1 h results in a loss of approximately half of activity, and nearly no activity is detected after preheating at 70┬░C for 1 h Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
2-oxoglutarate at pH 6.0 and 40┬░C Bacillus thuringiensis
0.089
-
L-isoleucine at pH 6.0 and 40┬░C Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus thuringiensis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 about 25% activity at pH 5.5 and 7.0, respectively, about 50% activity at pH 6.5 Bacillus thuringiensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the enzyme remains stable between pH 5.0 and 8.0 for 24 h Bacillus thuringiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.116
-
2-oxoglutarate at pH 6.0 and 40┬░C Bacillus thuringiensis
0.146
-
L-isoleucine at pH 6.0 and 40┬░C Bacillus thuringiensis