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Literature summary for 1.14.11.42 extracted from

  • Noma, A.; Ishitani, R.; Kato, M.; Nagao, A.; Nureki, O.; Suzuki, T.
    Expanding role of the jumonji C domain as an RNA hydroxylase (2010), J. Biol. Chem., 285, 34503-34507.
    View publication on PubMedView publication on EuropePMC
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Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ residues H160, N162, and H235 in the HX(D/E)XnH motif are required for Fe(II) binding Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-(3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + 2-oxoglutarate + O2 Homo sapiens enzyme catalyzes the hydroxylation of the hypermodified nucleoside wybutoside in position 37 of tRNAPhe. Residues Y106 and L175 are required for 2-oxoglutarate binding. H160, N162, and H235 are residues in the HX(D/E)XnH motif that are required for Fe(II) binding 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + succinate + CO2
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Organism

Organism UniProt Comment Textmining
Homo sapiens A2RUC4
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Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-(3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + 2-oxoglutarate + O2 enzyme catalyzes the hydroxylation of the hypermodified nucleoside wybutoside in position 37 of tRNAPhe. Residues Y106 and L175 are required for 2-oxoglutarate binding. H160, N162, and H235 are residues in the HX(D/E)XnH motif that are required for Fe(II) binding Homo sapiens 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + succinate + CO2
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additional information in the biogenesis of hydroxywybutosine, enzyme Tyw5p employs wybutosine residue 72 in tRNAPhe as a substrate by recognizing the N4-methyl group to hydroxylate the beta-carbon of the propyl group, followed by methylation and methoxycarbonylation of the side chain catalyzed by enzyme Tyw4 for completion Homo sapiens ?
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General Information

General Information Comment Organism
physiological function knock-down of enzyme gene by RNAi results in loss of hydroxywybutosine. Expression of the gene in yeast leads to formation of hydroxywybutosine. Enzyme Tyw5p employs wybutosine residue 72 in tRNAPhe as a substrate by recognizing the N4-methyl group to hydroxylate the beta-carbon of the propyl group, followed by methylation and methoxycarbonylation of the side chain catalyzed by enzyme Tyw4 for completion Homo sapiens